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Human embryonic primary cortical astrocytes

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Human embryonic primary cortical astrocytes are cells derived from the cerebral cortex of human embryos. They serve as a model for studying astrocyte biology and function in a human-relevant context.

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Primary human astrocytes (43 citations) Human astrocytes (43 citations) Primary human cortical astrocytes (4 citations) Human cortical astrocytes (2 citations)

2 protocols using human embryonic primary cortical astrocytes

1

Primary Cortical Astrocyte Culture

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Human embryonic primary cortical astrocytes were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured according to the manufacturer’s recommendations. Before experiments, characterization of the cells was performed to rule out the presence of microglia or neurons in the culture. All experiments were performed on cells in passage 4. Cells were serum starved (0.02% fetal bovine serum and 0.01% growth supplement mix) for 24 h before experiments. Cytokines and antibodies used in the cell studies are described in the Supplementary Information.
Sellgren C.M., Kegel M.E., Bergen S.E., Ekman C.J., Olsson S., Larsson M., Vawter M.P., Backlund L., Sullivan P.F., Sklar P., Smoller J.W., Magnusson P.K., Hultman C.M., Walther-Jallow L., Svensson C.I., Lichtenstein P., Schalling M., Engberg G., Erhardt S, & Landén M. (2015). A genome-wide association study of kynurenic acid in cerebrospinal fluid: implications for psychosis and cognitive impairment in bipolar disorder. Molecular Psychiatry, 21(10), 1342-1350.
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2

Culturing Primary Cortical Astrocytes

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Human embryonic primary cortical astrocytes were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) and cultured according to the manufacturer's recommendations. Before experiments, characterization of the cells was performed to rule out the presence of microglia or neurons in the culture. All experiments were performed on cells in passage 4. Cells were serum starved (0.02% fetal bovine serum and 0.01% growth supplement mix) for 24 h before experiments. Cytokines and antibodies used in the cell studies are described in the Supplementary Information.
Sellgren C.M., Kegel M.E., Bergen S.E., Ekman C.J., Olsson S., Larsson M., Vawter M.P., Backlund L., Sullivan P.F., Sklar P., Smoller J.W., Magnusson P.K., Hultman C.M., Walther-Jallow L., Svensson C.I., Lichtenstein P., Schalling M., Engberg G., Erhardt S, & Landén M. (2015). A genome-wide association study of kynurenic acid in cerebrospinal fluid: implications for psychosis and cognitive impairment in bipolar disorder. Molecular Psychiatry, 21(10), 1342-1350.
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