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Mouse u6 promoter

Manufactured by Thermo Fisher Scientific
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The Mouse U6 promoter is a DNA sequence used to drive the expression of small RNA molecules, such as short hairpin RNA (shRNA) or small interfering RNA (siRNA), in mouse cell lines. It provides a robust and consistent level of RNA transcription in a wide range of mouse cell types.

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Lab products found in correlation

Silencer sirna construction kit, by Thermo Fisher Scientific (1 mentions)

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U6 promoter

2 protocols using mouse u6 promoter

1

In vivo Gene Silencing by shRNA

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For in vivo gene silencing, TLR2-specific and IFN-γRα specific short hairpin oligos (shRNA~50 bases) were synthesized with a nine base loop sequences in the middle and a terminator sequence (five to six Ts) at the 3’-end and inserted in the multiple cloning site of pSilencer 1.0 U6 (mouse) plasmid vector having mouse U6 promoter (Ambion Inc., Grand Island, NY). Scrambled shRNA was used as Control shRNA.
Chowdhury B.P., Bandyopadhyay S., Das S., Majumder S., Jha M.K., Majumdar S.B., Saha B, & Majumdar S. (2015). The Host-Protective Effect of Arabinosylated Lipoarabinomannan against Leishmania donovani Infection Is Associated with Restoration of IFN-γ Responsiveness. PLoS ONE, 10(2), e0117247.
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2

CCR5-Specific siRNA and shRNA Synthesis

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We synthesized CCR5 specific small interfering RNA (siRNA) using the Silencer siRNA Construction kit (Ambion) [14] (link). GAPDH siRNA was used for negative control. For sustained gene silencing effect in vivo, short hairpin oligos (shRNA∼50 bases) were synthesized with the same sense and antisense sequences separated by a nine base loop sequences in the middle and a terminator sequence (five to six Ts) at the 3′-end and inserted in the polycloning site of pSilencer 1.0 U6 (mouse) plasmid vector having mouse U6 promoter (Ambion Inc.).
Das S., Banerjee S., Majumder S., Paul Chowdhury B., Goswami A., Halder K., Chakraborty U., Pal N.K, & Majumdar S. (2014). Immune Subversion by Mycobacterium tuberculosis through CCR5 Mediated Signaling: Involvement of IL-10. PLoS ONE, 9(4), e92477.
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