Esi q exactive orbitrap ms

Spirostanol saponins identification was carried out by using a Thermo ESI-Q-Exactive-Orbitrap MS (in the positive ESI mode (capillary voltage: 3.2 kV). Ultra-high purity nitrogen (N₂) and high purity nitrogen (N₂) were used as the collision gas and the sheath/auxiliary gas, respectively. The ESI source parameters were 350 °C for capillary temperature, 300 °C for ion source heater temperature, 40 arbitrary units for sheath gas (N₂), 10 arbitrary units for auxiliary gas (N₂), and the collision energy of the quadrupole ranged between 15 and 45 V were used. The mass range of the Orbitrap analyzer scanner was m/z 150 to 1500. Selected precursors analyzed more than two times were actively excluded from analysis for 60 s. Monitoring time was 0–65 min. Data recording and processing were performed using the Xcalibur 4.0 software (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The accuracy error threshold was fixed at 5 ppm. Automatic components extraction was accomplished by a Sieve v2.2 SP2 (Thermo Fisher Scientific) with the time range from 0 to 65 min and the BP minimum count at 10,000. Mass resolution is 70,000 and 17,500 for MS and MS², respectively.

To analyze and identify the chemical constituents in the extracts of SQYZD, we conducted a mass spectrometry analysis. 1 ml of 80% methanol was added to 200 μl SQYZD and vortexed for 5 min. Then, it was centrifuged at 4°C for 10 min at 12000 rpm. The supernatant was taken for ESI-Q-Exactive-Orbitrap MS in positive/negative ion mode (Thermo Fisher, USA). The data collected by high-resolution liquid quality were sorted preliminarily by CD 2.1 (Thermo Fisher, USA) and then retrieved and compared in MzCloud database.