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The 30A-GAL4 is a genetic tool used in Drosophila research. It is a transgenic fly line that expresses the yeast transcriptional activator GAL4 under the control of the 30A promoter. This allows for targeted gene expression in specific cell types or tissues of the Drosophila model organism.

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2 protocols using 30a gal4

1

Genetic Toolkit for Drosophila Research

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These stocks are described in Flybase: w1118, y1w1118, wgI-12, wgI-16(Df(2L)wg-CX3, wg[l-16] b[1 (link)] pr[1 (link)]/CyO), STAT92EF, STAT92E06346, ptc-GAL4 (on Ch II), en-GAL4 (on Ch II), rn-GAL4 (on Ch III), 30A- GAL4 (on Ch II, Bloomington stock#37534), Ptub-GAL80ts on Ch III, 10XSTAT-GFP, UAS-GFP (on Ch II), UAS-TCFDN (on Ch III), UAS-Axin-GFP (on Ch III), UAS-STATRNAi (on X), rpr-11-lacZ (on Ch III). The stock used for lineage tracing is also described in Flybase; w*; P{UAS-RedStinger}4, P{UAS-FLP.D}JD1, P{Ubi-p63E(FRT.STOP)Stinger}9F6 /CyO (Bloomington stock#28280). STAT92EF is a temperature-sensitive EMS-induced point mutation [62 (link)]. STAT92E06346 is a strong loss-of-function allele caused by a transposon-insertion that produces no transcript or protein [63 (link),64 (link)]. wg1-12 is an EMS-induced temperature sensitive allele [22 (link)].
Other stocks: DIAP1-lacZ [65 (link)], hid-GFP [31 (link)], ci-GAL4 [66 (link)], UAS-rpr (on X, [67 (link)]), UAS-STAT92E [30 (link)], rpr87 [32 (link)], and Df(3L)XR38 [68 (link)].
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2

Drosophila Genetic Toolkit for Research

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Stocks used in this study include the following: hsFLP; act<stop<Gal4, UAS-ykiS168A.v5, hsFLP;act<stop<Gal4, UAS-RFPNLS, hsFLP, UAS-GFPNLS; tub-Gal80, FRT40A (MARCM FRT 40A), ubi>RFP, FRT40A, banΔ1, banGFP, en-Gal4, dpp-lacZ, UAS-RFP, AP-1-RFP (53 (link)), and TIE-DYE (50 (link)). Stocks were obtained from the Bloomington Drosophila Stock Center (BDSC): UAS-ykiS168A.v5 (#28818), UAS-yki.v5 (#28819), UAS-yki::GFP (#28815), wtsX1 (#44251), smo3 (#3277), UAS-ciRNAi (#64928), tara1-lacZ (#6403), UAS-taraRNAi (#31634), nub-Gal4 (#42699), 30A-Gal4 (#37534), UAS-dMyc (#9674), UAS-rasv12 (#4847), and UAS-jnkDN (#9311). Ci-lacZ was a gift from D. Kalderon (Columbia University, USA). UAS-bantamsponge and UAS-bantam.D were gifts from S. Cohen (University of Copenhagen, Denmark). hs-Pc-sensor was a gift from V. Pirrotta (Rutgers University, USA). UAS-myc::tara was a gift from R. Smith-Bolton [University of Illinois, Urbana-Champaign, USA (originally from M. Cleary, University of California, Merced, USA)]. MARCM FRT19A and sd47M, FRT19A were gifts from D. Pan.
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