A total of 20 male C57BL/6J mice were purchased from Peking University Health Science Center (Beijing, China). Mice were randomly divided into two groups (n = 10 for each group), one was intraperitoneal injected with STZ dissolved in a low pH (pH 4.5) citrate buffer (50 mg/kg for 5 consecutive days) to build the DN models or citrate buffer alone. Hyperglycaemic state was monitored and maintained on a standard rodent diet with water ad libitum and tested with a glucose metre (Sinocare, China) according to the manufacturer’s instructions. At the end of 12 weeks after the onset of diabetes, the blood of the mice was collected from mice through submandibular vein and the serum was extracted for the following experiments. All animal protocols were in accordance with the standards of the Institutional Animal Care and Use Committee (IACUC). And All animal studies were performed based on the review and approval of Animal Ethics Committee of the First Affiliated Hospital of Guangxi Medical University (approval number: [KY-E-2020-01-25]).
Glucose metre
Manufactured by Sinocare
Sourced in China
The Glucose Meter is a compact, portable device designed to measure and display the glucose level in a small blood sample. It provides a quick and convenient way to monitor blood glucose levels, which is essential for managing conditions such as diabetes.
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Lab products found in correlation
2 protocols using glucose metre
1
Diabetes Nephropathy Development in Mice
2
Diabetic Mouse Model Characterization
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Eight-week-old male db/db diabetic mice and their non-diabetic littermate control db/m mice in the C57BL/KsJ background were acquired from the Model Animal Research Centre of Nanjing University (Nanjing, China). Fasting blood glucose was tested with a glucose metre (Sinocare, China) according to the manufacturer's instructions. Mice were placed in metabolic cages for 24-h urine collection with free access to food and water. The urine samples were centrifuged to pellet the debris, and supernatants were aliquoted and stored at -80°C for later detection. Quantitative urinary albumin was measured by using a mouse ELISA kit (Sigma, St. Louis, MO, USA). The experimental procedure was approved by the Animal Care and Use Committee of The First Affiliated Hospital of Soochow University, and all experimental animals were handled in agreement with the appropriate rules and regulations.
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