Universal mycoplasma detection kit 30 1012k

Manufactured by American Type Culture Collection

The Universal Mycoplasma Detection Kit #30–1012K is a laboratory tool designed to detect the presence of mycoplasma contamination in cell cultures. It provides a standardized and reliable method for the identification of various mycoplasma species.

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Lab products found in correlation

Powerplex 1.2 kit, by Promega (1 mentions) Ham s f12 medium, by Merck Group (1 mentions) Rpmi 1640, by Merck Group (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Hydrocortisone, by Merck Group (1 mentions) Nci h460, by American Type Culture Collection (1 mentions) Nci h209, by American Type Culture Collection (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Penicillin, by Merck Group (1 mentions) Streptomycin, by Merck Group (1 mentions)

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Universal Mycoplasma Detection Kit (395 citations) Mycoplasma detection kit (15 citations) Universal Detection Kit (4 citations)

4 protocols using universal mycoplasma detection kit 30 1012k

Neonatal Mouse Dermal Fibroblast Isolation and Human Skin Tumor Fibroblast Characterization

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Neonatal mouse dermal fibroblasts: Isolation was carried out essentially as described (Lichti et al., 2008 (link)), except that skin from limbs was used instead of trunk skin. Each neonate was genotyped using PCR prior to cell isolation and genotyping. Cells were cultured in DMEM with 10% FBS and antibiotics.
Human skin tumor fibroblasts: Biopsies used for cell culture were cut into pieces and placed into 35 mm culture dishes with enough DMEM with 10% FBS containing antibiotics to just cover the pieces. Adherent fibroblasts that migrated out were expanded and cryopreserved. Cell lines were all tested for tuberin (TSC2) levels and pS6 levels under serum-starved conditions by Western blot. Cells displaying decreased tuberin and TSC2 activation were used for analysis of Gal-3 levels. Mouse and human cells were free from detectable mycoplasma, using the ATCC Universal Mycoplasma Detection Kit #30–1012K.

Klover P.J., Thangapazham R.L., Kato J., Wang J.A., Anderson S.A., Hoffmann V., Steagall W.K., Li S., McCart E., Nathan N., Bernstock J.D., Wilkerson M.D., Dalgard C.L., Moss J, & Darling T.N. (2017). Tsc2 disruption in mesenchymal progenitors results in tumors with vascular anomalies overexpressing Lgals3. eLife, 6, e23202.

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Cell Line Maintenance and Manipulation for Breast Cancer Research

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MCF-DCIS cells were as described (27 (link)). MDA-MB-231 cells (from ATCC or the Whitehurst lab, UT Southwestern) were maintained in DMEM (Sigma-Aldrich, D5796) with 10% FBS (Sigma-Aldrich, F0926) and 1% penicillin/streptomycin (Thermo Fisher Scientific, SV30010). SUM159 (Asterand) cells were cultured in Ham’s F-12 medium (Sigma-Aldrich, N6658) with 5% FBS, 5 μg/mL insulin (Sigma-Aldrich, I0516), and 1 μg/mL hydrocortisone (Sigma-Aldrich, H0888). BT-549, BT-20, HCC1569, and HCC1419 lines were obtained from the Minna laboratory, UTSW, and grown in RPMI1640 (Sigma-Aldrich, R8758) with 5% FBS and 1% penicillin-streptomycin. Bone-derived metastatic (BOM) MDA-MB-231 cells were obtained from parental MDA-MB-231 as described (29 (link)). Cell lines were either fingerprinted (PowerPlex 1.2 Kit, Promega) or independently authenticated annually (ATCC, IDEXX Bioresearch) and mycoplasma-free (e-Myco Kit, Boca Scientific or Universal Mycoplasma Detection Kit 30-1012K, ATCC). All cells were maintained at 37°C and 5% CO₂. For immunoblotting, cells were grown to 60% confluency and treated with the indicated siRNA (OSR1 or WNK1) overnight and lysed before cells attained 75% confluency for immunoblotting. Cells were treated with inhibitors WNK463 or BGB324 overnight prior to lysing and immunoblotting.

Jaykumar A.B., Jung J.U., Parida P.K., Dang T.T., Wichaidit C., Kannangara A.R., Earnest S., Goldsmith E.J., Pearson G.W., Malladi S, & Cobb M.H. (2021). WNK1 Enhances Migration and Invasion in Breast Cancer Models. Molecular cancer therapeutics, 20(10), 1800-1808.

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Lung Cancer Cell Lines Authentication

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The non-small cell lung cancer (NSCLC) cell lines NCI-H460 (bio-69123) and small cell lung cancer (SCLC) cell lines NCI-H209 (bio-69576) were purchased from ATCC and authenticated by STR profiling. Both lung cancer cells were cultured in the RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) at 37 °C with 5% CO₂ humidified atmosphere. All cell lines were tested routinely for mycoplasma contamination with a universal mycoplasma detection kit (30–1012 K; American Type Culture Collection, Manassas (ATCC), VA).

A R., Wang H., Nie C., Han Z., Zhou M., Atinuke O.O., Wang K., Wang X., Liu S., Zhao J., Qiao W., Sun X., Wu L, & Sun X. (2023). Glycerol-weighted chemical exchange saturation transfer nanoprobes allow 19F/1H dual-modality magnetic resonance imaging-guided cancer radiotherapy. Nature Communications, 14, 6644.

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Transient Transfection of Cav1.3 Channels

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TsA-201 cells were obtained from the European Collection of Authenticated Cell Cultures (ECACC, catalogue number 96121229, lot number 13D034) at passage 6. Cell stocks of passage 8 were frozen and cultures were re-expanded from stocks for not more than 20 passages. Cell cultures were tested negative (Universal Mycoplasma Detection Kit 30-1012 K, American Type Culture Collection) for mycoplasma infection. For electrophysiological experiments tsA-201 cells were maintained and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, D6546) completed with 10% FBS (Gibco, 10270-106), 2 mm l-glutamine (Gibco, 25030-032), penicillin (10 U/ml; Sigma-Aldrich, P3032) and streptomycin (10 μg/ml; Sigma-Aldrich, S6501) at 37°C and 5% CO₂ in a humidified incubator. Cells were transiently transfected with 3 μg of WT or mutated Cav1.3 α1, 2 μg β2a (rat, M80545) (50 (link)) or β3 (rat, NM_012828) (50 (link)) and 2.5 μg α2δ-1 subunits (rabbit, NM_001082276) (50 (link)) using the Ca²⁺-phosphate precipitation method always including EGFP cDNA (1.5 μg) as a transfection marker. All data were obtained from at least 3 independent transfections.

Török F., Tezcan K., Filippini L., Fernández-Quintero M.L., Zanetti L., Liedl K.R., Drexel R.S., Striessnig J, & Ortner N.J. (2022). Germline de novo variant F747S extends the phenotypic spectrum of CACNA1D Ca2+ channelopathies. Human Molecular Genetics, 32(5), 847-859.

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