In situ hybridization was performed as described previously (46 (link)). Digoxigenin-labeled riboprobes were generated by in vitro transcription using 11-digoxigenin UTPs (Roche, Switzerland). Complementary DNA (cDNA) fragments of mouse Cyfip1, Cyfip2 and Fmr1 for in situ hybridization probes were obtained by PCR using a mouse P6 retinal cDNA library as a template. Primers used for amplification are listed in
11 digoxigenin utps
Manufactured by Roche
Sourced in Switzerland
The 11-digoxigenin UTPs are a set of nucleotide triphosphates that are modified with the addition of a digoxigenin label. These labeled nucleotides can be incorporated into nucleic acid probes or amplified products, allowing for their detection and visualization in various applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 11 digoxigenin utps
1
In situ Hybridization Probes for Cyfip1, Cyfip2, and Fmr1
Check if the same lab product or an alternative is used in the 5 most similar protocols
In situ hybridization was performed as described previously (46 (link)). Digoxigenin-labeled riboprobes were generated by in vitro transcription using 11-digoxigenin UTPs (Roche, Switzerland). Complementary DNA (cDNA) fragments of mouse Cyfip1, Cyfip2 and Fmr1 for in situ hybridization probes were obtained by PCR using a mouse P6 retinal cDNA library as a template. Primers used for amplification are listed inSupplementary Material, Table S1 .
+ Expand
In situ hybridization was performed as described previously (46 (link)). Digoxigenin-labeled riboprobes were generated by in vitro transcription using 11-digoxigenin UTPs (Roche, Switzerland). Complementary DNA (cDNA) fragments of mouse Cyfip1, Cyfip2 and Fmr1 for in situ hybridization probes were obtained by PCR using a mouse P6 retinal cDNA library as a template. Primers used for amplification are listed in
2
In Situ Hybridization of Retinal Genes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In situ hybridization was performed as described previously41 (link). In our current study, we modified the proteinase K treatment time from 5 to 10 min. The cDNA fragments of zebra finch Rhodopsin, S-opsin (cone opsin) and Arhgef33 were amplified by PCR from zebra finch retinal cDNA with the primers shown in Table S1 . Digoxigenin-labeled riboprobes for those genes were prepared by in vitro transcription with 11-digoxigenin UTPs (Roche).
3
Mef2 Family Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In situ hybridization was carried out as described previously (Muranishi et al. 2011) . Digoxigenin-labeled riboprobes for mouse Mef2 family genes were generated by in vitro transcription using 11-digoxigenin UTPs (Roche). We used probes containing fragments of Mef2a (821 bp), Mef2b (752 bp), Mef2c (764 bp) and Mef2d (1079 bp) cDNAs, which were amplified from P0 mouse retinal cDNAs. The primer sequences are listed in Supporting Information Table S3.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!