MDSCs and slices of rats were fixed using 4% paraformaldehyde (Servicebio, Wuhan, China) and permeabilized using 0.05% TritonX-100 (Servicebio). After blocking, MDSCs and slices of rats were stained with primary antibodies against α-SMA (Cell Signaling Technology) at 4°C overnight. Then they were incubated with FITC anti-mouse IgG (Proteintech, Rosemont, IL, USA) for 1 h. After washing three times with PBA, slices were stained with diamidino-phenyl-indole (DAPI) to detect the nuclei. Images were captured using a laser scanning confocal microscope (Leica TCS SP5, Wetzlar, Germany).
Fitc anti mouse igg
Manufactured by Proteintech
Sourced in United States, Germany
FITC anti-mouse IgG is a fluorescently-labeled secondary antibody that binds to mouse immunoglobulin G (IgG) molecules. It is designed for use in various immunodetection techniques, such as flow cytometry, immunofluorescence, and Western blotting, to visualize and detect the presence of mouse IgG proteins in biological samples.
Automatically generated - may contain errors
2 protocols using fitc anti mouse igg
1
Immunofluorescent Staining of MDSCs
2
Immunohistochemical Analysis of Hepatic Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The sections of liver tissues were fixed in 4% paraformaldehyde for 30 min and then permeabilized in 0.05% TritonX-100 for 10 min. The sections were stained with primary antibodies against CD68 (CST) and IL-1β (CST) at 4°C overnight, followed by the blocking with 1% BSA for 1 h. After washing with PBS three times, sections was stained with FITC anti-mouse IgG (Proteintech) and Cy3 anti-rabbit IgG (Proteintech) for 1 h. A laser scanning confocal microscope (Leica, Wetzlar, Germany) was used to capture the images.
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