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Cd56 aa700

Manufactured by Beckman Coulter
Sourced in United States

The CD56-AA700 is a fluorescent-labeled antibody used in flow cytometry analysis. It binds to the CD56 antigen, which is expressed on natural killer cells and a subset of T cells. The CD56-AA700 antibody is conjugated to the Alexa Fluor 700 fluorescent dye, allowing for its detection in the far-red region of the spectrum.

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2 protocols using cd56 aa700

1

Circulating Tumor Plasma Cell Detection

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Circulating tumor plasma cells in the peripheral blood (PB) were detected using flow cytometry. Briefly, PB samples were collected in tubes containing EDTA and processed using a bulk-lysis procedure (Versalyse, Beckman Coulter, Brea, CA, USA) within 24 h of sample collection. Labeling was performed using a single 8-colors antibody combination: CD45-KrOr, CD138-PC5.5, CD56-AA700, CD27-PC7, CD19-ECD, CD200-AA750 (Beckman Coulter), Kappa-APC, and Lambda FITC (BD Biosciences, Franklin Lakes, NJ, USA), and CD38-BV421 (Biolegend, San Diego, CA, USA). First, membrane staining was performed and this was followed after a PBS washing procedure by intracytoplasmic labeling (anti-Kappa and anti-Lambda) using a Fix & Perm Cell Permeabilization Kit (Beckman Coulter, Brea, CA, USA). After the final PBS wash, the final cell pellet was resuspended in 500 µL of PBS before analysis using the flow cytometer (Navios, Beckman Coulter, Brea, CA, USA). From 200,000 to 1 million leucocytes were acquired for each patient to ensure the quality of the analysis and to allow a limit of detection to be set at ≥20 tumor plasma cells (0.01% to 0.002%, according to the number of leucocytes). Data were analyzed using Kaluza 2.1 software (Beckman Coulter, Brea, CA, USA).
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2

Multiparameter Flow Cytometry Analysis

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Blood samples were stained with the following fluorochrome-conjugated antibodies: MDC8-FITC, CD303-PEVio770, (Miltenyi Biotec), CD45 Krome Orange, CD14-Pacific Blue, CD3-AA750, CD19-AA750, CD56-AA700, CD123-ECD, CD1c PE/Cy5.5 (from Beckman Coulter), HLA-DR-Super Bright 600, CD16-BV785 (Biolegend), OX40L-PE or isotypic control (Biolegend), CD274 (PDL1) APC (BD Biosciences). For Tfh cell analysis, the following-conjugated antibodies were used: CD3-A750, CD4-Pacific Blue (Beckman Coulter); CD45-FITC, CD45RA-PE/Dazzle, CXCR5-PE/Cy7 (Biolegend); OX40-PE (Miltenyi Biotec). Samples were stained with the antibodies for 15 min and then incubated for 15 min with 1 ml BD FACS lysis solution, washed, and subsequently analyzed by flow cytometry. Compensation beads were used for compensation settings (VersaComp; Beckman Coulter). Cells were analyzed on a Cytoflex flow cytometer (Beckman Coulter). Data were analyzed using Kaluza V5.1 software (Beckman Coulter).
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