Rabbit α muc2

Rat tail type I collagen, Transwell™ inserts, 12-well polystyrene tissue culture dishes, and 12-well polycarbonate filters were purchased from Corning (Corning, NY). Human collagen (type I) was obtained from Advanced Biomatrix (Carlsbad, CA). Collagenase (type IV) was from Worthington Biochemicals (Lakewood, NJ). Gastrin was obtained from Anaspec (Freemont, CA). N-acetyl cysteine was from MP Biomedicals (Santa Ana, CA). Murine EGF was obtained from Peprotech (Rock Hill, NJ). Primocin was purchased from InvivoGen (San Diego, CA). SB202190 was obtained from Selleckchem (Houston, TX). Nicotinamide, A83–01, fluorescein-dextran (70 kDa), atenolol, metoprolol, propranolol, prazosin, doxazosin, digoxin, zosuquidar, and digitoxin were acquired from Sigma-Aldrich (St. Louis, MO). Y-27632 and N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) was from ApexBio Technology (Houston, TX). Red alkaline phosphatase substrate kit was obtained from Vector Labs (Burlingame, CA). Rabbit α-Muc2, ZO-1, and integrin β4 were obtained from Santa Cruz Biotechnology (Dallas, TX). Donkey serum and donkey anti-rabbit or mouse IgG conjugated with Alexa Fluor 488 were from Jackson Immunoresearch (West Grove, PA). Ko143 was purchased from Cayman Chemical (Ann Arbor, MI). All other reagents were from Thermo Fisher Scientific (Waltham, MA).