To induce neurotoxicity, SH‐SY5Y cells were exposed to 25 μM of 6‐OHDA (Sigma) for 24 h. Prior to 6‐OHDA exposure, SH‐SY5Y cells were transfected with plasmids carrying PCBP1 and empty vectors using Lipofectamine 3000 (Invitrogen) 24 h before the 6‐OHDA treatment. After 36 h, the cell growth of SH‐SY5Y cells was assessed as described below.
6 ohda
6-OHDA is a chemical compound used as a tool in neuroscience research. It is a selective neurotoxin that can induce degeneration of dopaminergic neurons, which are important for the study of Parkinson's disease and other neurological disorders. The core function of 6-OHDA is to serve as a research tool for investigating the mechanisms and effects of dopaminergic neuron loss.
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2 protocols using 6 ohda
SH-SY5Y Cells Overexpressing PCBP1 Resist 6-OHDA Toxicity
To induce neurotoxicity, SH‐SY5Y cells were exposed to 25 μM of 6‐OHDA (Sigma) for 24 h. Prior to 6‐OHDA exposure, SH‐SY5Y cells were transfected with plasmids carrying PCBP1 and empty vectors using Lipofectamine 3000 (Invitrogen) 24 h before the 6‐OHDA treatment. After 36 h, the cell growth of SH‐SY5Y cells was assessed as described below.
Neuroprotective Effects of Natural Compounds
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