Hucct 1

Atorvastatin and gemcitabine were purchased from Sawai Pharmaceutical Co. Ltd. (Osaka, Japan) and Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan), respectively. HuCCT-1 and KKU-M213 cells were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). HuCCT-1 cells were cultured in Roswell Park Memorial Institute 1640 medium (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum and 1% ampicillin/streptomycin. KKU-M213 cells were cultured in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific K.K., Kanagawa, Japan) supplemented with 10% fetal bovine serum and 1% ampicillin/streptomycin.

HEK293 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HuCCT1 and HEL cells were purchased from the Japanese Cancer Research Resources Bank (Japan). BM-hMSCs and hPBMCs were purchased from Lonza Group AG (Switzerland). HEK293 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) high-glucose GlutaMAX supplement (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% penicillin-streptomycin (Nacalai Tesque). HuCCT1 and HEL cells were maintained in RPMI 1640 medium (Nacalai Tesque) supplemented with 10% FBS and 1% penicillin-streptomycin. BM-hMSCs were maintained in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS and penicillin-streptomycin.