Anti cd8 pe clone rpa t8

Manufactured by BD

Sourced in United States

Anti-CD8-PE (clone RPA-T8) is a flow cytometry reagent that binds to the CD8 surface antigen expressed on a subset of T cells. It is conjugated with the fluorescent dye phycoerythrin (PE), allowing for the identification and enumeration of CD8+ T cells in samples using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd4 fitc clone rpa t4, by BD (2 mentions) Facscalibur, by BD (1 mentions) Anti cd3 alexa fluor 700 clone sp34.2, by BD (1 mentions) Anti cd4 percp clone l200, by BD (1 mentions) Celltrace cfse cell proliferation kit, by Thermo Fisher Scientific (1 mentions) Facsdiva, by BD (1 mentions)

3 protocols using anti cd8 pe clone rpa t8

Phenotypic Characterization of Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell surface markers of human MoDC, murine DC, or murine spleens were analyzed by FACS using the following antibodies: anti-HLA-DR-FITC, anti-CD45-PerCP, anti-CD14-PE, and anti-CD86-V450 (clone 2331) for human MoDC. For cell surface markers of murine DC, we used, biotin anti-IAb (clone AF6-120-1), anti-CD11c-PE (clone HL3), anti-CD40-APC (monoclonal 3/23 from BD Pharmingen), and anti-CD86-V450 (clone GL-1) and for murine spleens we also used anti-CD4-FITC (clone RPA-T4) and anti-CD8-PE (clone RPA-T8) (BD Biosciences). Data were analyzed using the FlowJo software. ANOVA was applied to these samples according to the manufacturer's instructions.

Teran-Navarro H., Salcines-Cuevas D., Calderon-Gonzalez R., Tobes R., Calvo-Montes J., Pérez-Del Molino Bernal I.C., Yañez-Diaz S., Fresno M, & Alvarez-Dominguez C. (2021). A Comparison Between Recombinant Listeria GAPDH Proteins and GAPDH Encoding mRNA Conjugated to Lipids as Cross-Reactive Vaccines for Listeria, Mycobacterium, and Streptococcus. Frontiers in Immunology, 12, 632304.

+ Open protocol

+ Expand

Murine DC and Spleen Cell Phenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell surface markers of murine DC or murine spleens were analyzed by FACS using the following antibodies: anti-HLA-DR-FITC, anti-CD45-PerCP, anti-CD14-PE and anti-CD86-V450 (clone 2331). For cell surface markers of murine DC, we used biotin anti-IAb (clone AF6-120-1), anti-CD11c-PE (clone HL3), anti-CD40-APC (monoclonal 3/23 from BD Pharmingen (BD Biosciences, San Jose, CA, USA)), anti-CD86-V450 (clone GL-1) and for murine spleens we also used anti-CD4-FITC (clone RPA-T4) and anti-CD8-PE (clone RPA-T8) (BD Biosciences). All samples were treated with propidium iodide to gate dead cells. Flow cytometry was performed with a FACSCalibur (BD Biosciences, San Jose, CA, USA) and data were analyzed using the FlowJo software.

Salcines-Cuevas D., Terán-Navarro H., Calderón-Gonzalez R., Torres-Rodriguez P., Tobes R., Fresno M., Calvo-Montes J., Molino-Bernal I.C., Yañez-Diaz S, & Alvarez-Dominguez C. (2021). Glyceraldehyde-3-phosphate Dehydrogenase Common Peptides of Listeria monocytogenes, Mycobacterium marinum and Streptococcus pneumoniae as Universal Vaccines. Vaccines, 9(3), 269.

+ Open protocol

+ Expand

CFSE-based SIV Gag Proliferation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMC were stained with CFSE (CellTrace^™ CFSE Cell Proliferation Kit, Invitrogen) and incubated with or without SIVmac239 Gag peptides (2 μg/ml for each peptide). The peptides (obtained through ARRRP) were 15-mers with an 11-amino acid overlap between sequential peptides and represented the complete protein sequence. Cells without any stimuli were used to determine background proliferation. After incubation for 5 days at 37 °C, cells were stained with anti-CD3-Alexa Fluor 700 (clone SP34-2), anti-CD4-PerCP (clone L200), and anti-CD8-PE (clone RPA-T8) Abs (all from BD Pharmingen). After fixation, at least 10,000 CD3⁺ cells were acquired by flow cytometry, and data were analyzed using FACS-Diva (BD Biosciences) software. The percentages of proliferating CD3⁺CD4⁺ and CD3⁺CD8⁺ cells were determined by CFSE dilution; background proliferation (without stimulation) was subtracted.

Sholukh A.M., Watkins J.D., Vyas H.K., Gupta S., Lakhashe S.K., Thorat S., Zhou M., Hemashettar G., Bachler B.C., Forthal D.N., Villinger F., Sattentau Q.J., Weiss R.A., Agatic G., Corti D., Lanzavecchia A., Heeney J.L, & Ruprecht R.M. (2015). Defense-in-depth by mucosally administered anti-HIV dimeric IgA2 and systemic IgG1 mAbs: Complete protection of rhesus monkeys from mucosal SHIV challenge. Vaccine, 33(17), 2086-2095.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!