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Anti cd8 pe clone rpa t8

Manufactured by BD
Sourced in United States

Anti-CD8-PE (clone RPA-T8) is a flow cytometry reagent that binds to the CD8 surface antigen expressed on a subset of T cells. It is conjugated with the fluorescent dye phycoerythrin (PE), allowing for the identification and enumeration of CD8+ T cells in samples using flow cytometry.

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3 protocols using anti cd8 pe clone rpa t8

1

Phenotypic Characterization of Dendritic Cells

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Cell surface markers of human MoDC, murine DC, or murine spleens were analyzed by FACS using the following antibodies: anti-HLA-DR-FITC, anti-CD45-PerCP, anti-CD14-PE, and anti-CD86-V450 (clone 2331) for human MoDC. For cell surface markers of murine DC, we used, biotin anti-IAb (clone AF6-120-1), anti-CD11c-PE (clone HL3), anti-CD40-APC (monoclonal 3/23 from BD Pharmingen), and anti-CD86-V450 (clone GL-1) and for murine spleens we also used anti-CD4-FITC (clone RPA-T4) and anti-CD8-PE (clone RPA-T8) (BD Biosciences). Data were analyzed using the FlowJo software. ANOVA was applied to these samples according to the manufacturer's instructions.
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2

Murine DC and Spleen Cell Phenotyping

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Cell surface markers of murine DC or murine spleens were analyzed by FACS using the following antibodies: anti-HLA-DR-FITC, anti-CD45-PerCP, anti-CD14-PE and anti-CD86-V450 (clone 2331). For cell surface markers of murine DC, we used biotin anti-IAb (clone AF6-120-1), anti-CD11c-PE (clone HL3), anti-CD40-APC (monoclonal 3/23 from BD Pharmingen (BD Biosciences, San Jose, CA, USA)), anti-CD86-V450 (clone GL-1) and for murine spleens we also used anti-CD4-FITC (clone RPA-T4) and anti-CD8-PE (clone RPA-T8) (BD Biosciences). All samples were treated with propidium iodide to gate dead cells. Flow cytometry was performed with a FACSCalibur (BD Biosciences, San Jose, CA, USA) and data were analyzed using the FlowJo software.
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3

CFSE-based SIV Gag Proliferation Assay

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PBMC were stained with CFSE (CellTrace CFSE Cell Proliferation Kit, Invitrogen) and incubated with or without SIVmac239 Gag peptides (2 μg/ml for each peptide). The peptides (obtained through ARRRP) were 15-mers with an 11-amino acid overlap between sequential peptides and represented the complete protein sequence. Cells without any stimuli were used to determine background proliferation. After incubation for 5 days at 37 °C, cells were stained with anti-CD3-Alexa Fluor 700 (clone SP34-2), anti-CD4-PerCP (clone L200), and anti-CD8-PE (clone RPA-T8) Abs (all from BD Pharmingen). After fixation, at least 10,000 CD3+ cells were acquired by flow cytometry, and data were analyzed using FACS-Diva (BD Biosciences) software. The percentages of proliferating CD3+CD4+ and CD3+CD8+ cells were determined by CFSE dilution; background proliferation (without stimulation) was subtracted.
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