Lcms 2010 ev liquid chromatograph mass spectrometer

HPLC-DAD-ESI/MS conducted the chemical analysis of steviol glycosides. A Shimadzu (Kyoto, Japan) LCMS-2010 EV Liquid Chromatograph Mass Spectrometer instrument was used with the LCMS solution version 3.0 software consisting of an SIL-20A prominence autosampler and an SPD-M20A diode array detector. These compartments were in conjunction with a mass selective detector that was equipped with an atmospheric pressure ionization. The HPLC separation (three replicates were analyzed) was accomplished on a Zorbax Eclipse Plus, 3.5 μm, 150 × 2.6 mm i.d. chromatographic column. The mobile phase solvents composed of: (A), 0.1% formic acid in water, (B), 0.1% formic acid in acetonitrile, and (C), Methanol. The flow rate was set at 0.3 mL min.⁻¹ and the mobile phase was identical with the one mentioned in the literature [53 (link)], with modification in the gradient, since not all steviol glycosides were included. The overall runtime was established at 25 min. Electron Spray Ionization (ESI) mode, using four distinct events for each analyte (see Table S1 and Figures S2 and S3), was applied in the selected ion monitoring mode (SIM). Photodiode array monitored wavelengths in the range of 190–800 nm.

A Shimadzu (Kyoto, Japan) LCMS-2010 EV Liquid Chromatograph Mass Spectrometer instrument was used with the LCMS solution version 3.0 software consisting of a SIL-20A prominence autosampler and an SPD-M20A diode array detector. The latter were coupled in series with a mass selective detector equipped with an atmospheric pressure ionization. The LC separation was achieved on a Zorbax Eclipse Plus, 3.5 μm, 150 × 2.6 mm i.d. chromatographic column (Agilent Technologies, Santa Clara, CA, USA). The mobile phase consisted of two channels, channel: 0.1% formic acid in water (A) and ACN (B). The flow rate and mobile phase gradient were identical to previously published work [110 (link)]. Similarly, the validation of the analytical method and quantitation of analytes was grounded on the previous work of our group [110 (link)], adapting the same sample preparation followed for the UHPLC-HRMS analysis.