Phosphate buffer saline tablets

HA used in this work was produced by Novozymes Biopharma A/S by fermentation of the novel, superior, and safe strain Bacillus subtilis. Molecular weight of the HA material was in the range of 0.7 to 1.0 MDa as determined by size exclusion chromatography combined with multiangle laser light scattering detection (SEC-MALS).
DVS was obtained from Merck Gmbh and Sigma Aldrich Co. Hyaluronidase (HAase) from bovine testes was purchased from Sigma Aldrich Denmark A/S (ref. H3506). Phosphate buffer saline (PBS) tablets without calcium and magnesium were obtained from MP Biomedicals Inc. (France).

Hyaluronic acid (HA) with a weight-average molecular weight (MW) of Low (L) 200, Medium (M) 500 and High (H) 1435 kDa were kindly provided by Altergon Italia s.r.l. Phosphate buffer saline (PBS) tablets without calcium and magnesium were obtained from MP Biomedicals Inc. hUCMSCs cells were extracted from the Wharton jelly of the umbilical cord kindly gifted by Ospedale Evangelico Betania (Naples, Italy), MRC-5 cells were purchased from ATCC. Dulbecco’s Modified Eagle′s—Medium (DMEM) (Microgem, Naples, Italy). SAGM (Lonza C-41 24) and Fetal Bovine Serum (FBS) were purchased from Lonza (Basel, Switzerland). Penicillin, streptomycin (10,000 U/ml) from Invitrogen and Life Technologies (Carlsbad, CA, USA) were employed. Trypsin and Ethylenediaminetetraacetic acid (EDTA) were from HiMedia (Mumbai, India). Formalin, bovine serum albumin (BSA) and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). SPC rabbit antibody from Abcam. FITC-conjugated anti-rabbit antibody (Millipore, Billerica, MA, USA). CD-73 antibody (Sigma Aldrich, St. Louis, MO, USA). Human pulmonary surfactant associated protein (SP) SP-A, SP-B, SPC and D ELISA kits were obtained from Elabscience.

Commercialized PSF (Udel®P-3500) was kindly supplied by Solvay (Brussels, Belgium). Ethanol and DMF with analytical grade were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). The powder of CSs was provided from local suppliers. Concentrated sulfuric acid (H₂SO₄, 98%) was brought from Nanjing Chemical Reagent Co., Ltd (Nanjing, China). Bovine serum albumin (BSA, 67 kDa) was obtained from Aladdin (Shanghai, China). Phosphate buffer saline (PBS) tablets were brought from MP Biomedicals LLC (Santa Ana, CA, USA). All chemicals were used without further purification. Deionized water was applied in all experiments.