Fluorescent microscopy was performed using an Axio Imager A2 compound microscope with a 63× lens (Zeiss, Oberkochen, Germany). Worms were prepared in an M9 buffer containing 2.5 mM levamisole on the slide glass in order to observe and take pictures. The GFP expression or intensity was measured using the ImageJ program (https://imagej.nih.gov/ij/ ). We also used the Lionheart FX automated microscope to compare GFP expression by quantity. Acquired images by Lionheart FX on a 40× lens on the slide glass were analyzed fluorescent quantity using the Gen5 software provided by the manufacturer. Statistical analysis in this study, such as standard deviation (SD), standard error (SEM) and t-test were performed using Sigmaplot (Systat software). P values *, **, *** were defined as <0.05, <0.01, and <0.001 respectively.
Axioimager a2 compound microscope
Manufactured by Zeiss
Sourced in Germany
The AxioImager A2 is a compound microscope designed for high-performance imaging and analysis. It features advanced optics, a stable and ergonomic design, and a variety of accessories to support a range of research and industrial applications.
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3 protocols using axioimager a2 compound microscope
1
Quantification of GFP expression in C. elegans
2
Mite Specimen Preparation and Identification Protocol
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Mites were collected from samples taken from decaying trees and cow dung, using Berlese funnels. They were posteriorly cleared in lactic acid and mounted in Hoyer's medium. The terminology for the idiosoma and legs follows that of Lindquist (1986) (link); the nomenclature of subcapitular setae and the designation of cheliceral setae follow those of Grandjean (1944 Grandjean ( , 1947)) , respectively. The systematics of Pygmephoroidea follows that of Khaustov (2004 Khaustov ( , 2008a)) . All measurements are given in micrometers (μm) for the holotype and paratypes (in parentheses). For leg chaetotaxy, the number of solenidia is given in parentheses. Mite morphology was studied using a Carl Zeiss AxioImager A2 compound microscope with phase contrast and DIC illumination. Photomicrographs were taken with Hitachi KP-HD20A digital camera.
Abbreviations: ap1-ap5 apodemes 1-5, appr prosternal apodeme, appo poststernal apodeme, apsej sejugal apodeme, Tr trochanter, Fe femur, Ge genu, Ti tibia, Ta tarsus, TiTa tibiotarsus, ass accessory setigenous structure, sol solenidion, ags anterior genital sclerite, pgs posterior genital sclerite, mgs median genital sclerite, php 1-3 pharyngeal pumps 1-3.
Abbreviations: ap1-ap5 apodemes 1-5, appr prosternal apodeme, appo poststernal apodeme, apsej sejugal apodeme, Tr trochanter, Fe femur, Ge genu, Ti tibia, Ta tarsus, TiTa tibiotarsus, ass accessory setigenous structure, sol solenidion, ags anterior genital sclerite, pgs posterior genital sclerite, mgs median genital sclerite, php 1-3 pharyngeal pumps 1-3.
3
Mite Identification Protocol via Microscopy
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Mites were collected from the soil samples by Berlese-Tullgren funnels. Collected mites were mounted in Hoyer's medium. Notation applied to the body and leg setae follow that of Grandjean's system, overviewed by Kethley (1990) and Norton (1977) applied to Caligonellidae by Swift (2001) ; palpal setation follows Grandjean (1946) . Mite morphology was studied using an AxioImager A2 compound microscope (Carl Zeiss, Germany) with phase-contrast and differential interference contrast (DIC) illumination. Photomicrographs were taken with an AxioCam ICc5 digital camera. All measurements are given in micrometers (μm) for holotype and available paratypes (in parentheses). For leg chaetotaxy, the number of solenidia is given in parentheses.
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