dLN were harvested directly into RNALater (Thermo Fisher Scientific, Waltham, MA) then RNA was isolated using the RNeasy Plus mini kit (Qiagen, Hilden, Germany). Any residual genomic DNA was then eliminated using the DNase away kit (Thermo Fisher Scientific, Waltham, MA). A cDNA library was then prepared using the RT kit (Thermo Fisher Scientific, Waltham, MA). qPCR was performed on CCL21 and HPRT using TaqMan primers (Applied Biosysems) and 2x qPCR master mix (Thermo Fisher Scientific, Waltham, MA) diluted with water and 250 ng of RNA from each sample. Relative expression of CCL21 in relation to HPRT was then calculated for each sample using the delta Ct method. All kits were used according to manufacturer’s instructions.
Dnase away kit
Manufactured by Thermo Fisher Scientific
The DNase Away kit is a cleaning solution designed to remove DNase contamination from laboratory equipment and surfaces. It effectively inactivates and denatures DNase enzymes, helping to ensure the integrity of DNA samples during experimental procedures.
Automatically generated - may contain errors
2 protocols using dnase away kit
1
Quantitative Analysis of CCL21 Expression
2
Quantitative Analysis of CCL21 Expression
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