Total drp1

Manufactured by Cell Signaling Technology

Sourced in United States

Total-DRP1 is an ELISA kit designed to quantitatively measure the total levels of Dynamin-related protein 1 (DRP1) in cell and tissue samples. DRP1 is a key regulator of mitochondrial fission, a process involved in various cellular functions. The Total-DRP1 ELISA provides a reliable and reproducible method for assessing the overall expression of DRP1 in experimental models.

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Lab products found in correlation

Total gsk3β, by Cell Signaling Technology (1 mentions) P creb, by Cell Signaling Technology (1 mentions) P gsk3β, by Cell Signaling Technology (1 mentions) Pgc 1α, by Abcam (1 mentions) Total creb, by Cell Signaling Technology (1 mentions) P drp1, by Cell Signaling Technology (1 mentions) Erk1 2, by Merck Group (1 mentions) Chemiluminescence substance, by Thermo Fisher Scientific (1 mentions) Mitofusin 2, by Abcam (1 mentions)

2 protocols using total drp1

Quantitative Western Blot Analysis

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Western blots were performed using antibodies to p-PKA substrate, p-CREB, total-CREB, p-DRP1, total-DRP1, p-GSK3β, total-GSK3β (Cell Signaling, Danvers, USA), UCP1, ERK1/2, Actin (Sigma, St. Louis, MO), PGC1α (Abcam, Cambridge, UK), OPA-1, MFN2 (Proteintech, Rosemont, USA), and home-made antibodies against DsbA-L. Quantification of Western blot results was conducted using NIH Image J software (National Institutes of Health, Bethesda, Md, USA).

Zhou H., Peng X., Hu J., Wang L., Luo H., Zhang J., Zhang Y., Li G., Ji Y., Zhang J., Bai J., Liu M., Zhou Z, & Liu F. (2021). DsbA-L deficiency in T cells promotes diet-induced thermogenesis through suppressing IFN-γ production. Nature Communications, 12, 326.

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Western Blotting of Mitochondrial Proteins

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Primary mouse hepatocytes were washed with cold PBS, collected in lysis buffer, sonicated, centrifuged (10,000xg for 15 minutes) and then the supernatant transferred to a new tube. Protein concentrations were determined using the BCA protein assay kit (Pierce). Samples were then mixed with loading buffer and run on a SDS-polyacrylamide gel. This gel was then transferred to a cellulose membrane. The membrane was blocked in 5% milk for one hour and then incubated in primary antibodies. Antibodies utilized were phosphorylated (serine 656) dynamin related protein 1 (DRP-1; Cell Signaling, Danvers, MA), total DRP-1 (Cell Signaling Technology, Danvers, MA), and Mitofusin-2 (Abcam, San Francisco, CA). Membranes were then washed in TBS-Tween 20 (TBST) for 30 minutes then placed in secondary antibody for one hour and then washed for one hour in TBST prior to being developed using chemiluminescence substance (Thermo Scientific, Rockford, IL).

Kautza B., Gomez H., Escobar D., Corey C., Ataya B., Luciano J., Botero A.M., Gordon L., Brumfield J., Martinez S., Holder A., Ogundele O., Pinsky M., Shiva S, & Zuckerbraun B.S. (2015). Inhaled, Nebulized Sodium Nitrite Protects In Murine and Porcine Experimental Models Of Hemorrhagic Shock and Resuscitation by Limiting Mitochondrial Injury. Nitric oxide : biology and chemistry, 51, 7-18.

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