Optical rotations were performed using an ATAGO AP-300 automatic polarimeter. UV and IR were obtained using PerkinElmer Lambda 35 UV/VIS Double Beam and Everest ATR Thermos scientific FTIR spectrometers. ECD were recorded on a JASCO J-720 CD spectrometer. Furthermore, high-resolution mass spectra (HR-MS) were determined on a Waters Xevo Q-TOF direct probe/MS system, using ESI+ mode and microchannel plates MCPs detector (Milford, MA, USA). JEOL ECZ and Bruker Topspin spectrometers at 500 MHz for 1H and 125 MHz for 13C NMR were used to measure NMR spectra, using TMS as an internal standard. The reference to the solvent of CDCl3 were assigned in δ (ppm) with signals (δH 7.26, δC 77.16). Additionally, column chromatography (CC) was subjected using silica gel 60 (Merck, 70–230 and 230–400 mesh) and octa desylsilane (Fuji sylisia chemical LTD, Chromatorex® C18 DM1020 M, 100–200 mesh). Thin-layer chromatography (TLC) precoated silica gel 60 F254 (Merck) and RP-18 F254s plates (Merck) were used to guide the separation process while UV light at 254 and 365 nm was employed for detection before spraying with 10% H2SO4 in ethanol.
Ap 300 automatic polarimeter
Manufactured by Atago
Sourced in United States
The AP-300 automatic polarimeter is a laboratory instrument designed to measure the optical rotation of a sample. It determines the angle of rotation of plane-polarized light passing through a sample, providing a precise measurement of the sample's optical activity. The AP-300 automates the polarimetric analysis process, offering accurate and reproducible results.
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Lab products found in correlation
Rp 18 f254s plates, by Merck Group (1 mentions)
J 720 cd spectrometer, by Jasco (1 mentions)
Mcps detector, by Waters Corporation (1 mentions)
Xevo q tof direct probe ms system, by Waters Corporation (1 mentions)
Precoated silica gel 60 f254 plates, by Merck Group (1 mentions)
Sephadex lh 20, by Merck Group (1 mentions)
Silica gel 60, by Merck Group (1 mentions)
3 protocols using ap 300 automatic polarimeter
1
Comprehensive Characterization of Organic Compounds
2
Measuring Optical Rotation of Compounds
3
Analytical Techniques for Metabolite Characterization
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Silica gel 60 (Merck, Darmstadt, Germany) and Sephadex LH-20 (Sigma-Aldrich, Steinheim, Germany), as well as pre-coated Silica gel 60 F254 plates (Merck, Darmstadt, Germany), were used for column chromatography (CC) and thin-layer chromatography (TLC), respectively. 1H and 13C NMR data of isolated metabolites were measured on Bruker Avance (Bruker, Rheinstetten, Germany) at 400, 500, and 600 MHz for 1H NMR, while 100, 125, and 150 MHz for 13C NMR. The NMR data were represented as δ ppm values relative to the internal reference TMS. ESI-MS spectra were recorded on a Micromass AC-TOF spectrometer (Micromass, Agilent Technologies 1,200 series, Waldbronn, Germany). UV data were reported on a UV-, V-630 BIO spectrophotometer (JASCO, United States). Specific optical rotation was measured on Ap-300 Automatic polarimeter (ATAGO Co. Ltd., United States). All chemicals and solvents used were of analytical grade.
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