The primary antibodies used included mouse anti-Nup153, ascites fluid (gift from B. Burke), rabbit anti-Nup153 (raised against hNup153-GST, amino acids 1300–1399), rabbit anti-Parp (9542, Cell Signaling), mouse anti-Oct-3/4 (sc-5279, Santa Cruz Biotechnology), goat anti-Oct-3/4 (sc-8628, Santa Cruz Biotechnology), mouse anti-α-tubulin (T5168, Sigma-Aldrich), mouse anti-Flag (F1804, Sigma-Aldrich), rabbit anti-Nup107 (raised against mNup107-His, amino acids 600–926), rabbit anti-Tuj1 (neuron-specific class III β-Tubulin; PRB-435P, BioLegend), mouse anti-Gapdh (GTX627408, Genetex), rabbit anti-Blbp (Fabp7; ab32423, Abcam), rat anti-Nestin (556309, BD pharmigen), mouse anti-Gata4 (sc-25310, Santa Cruz Biotechnology), rabbit anti-GFAP (Z0334, Dako), goat anti-Foxa2 (AF2400, R&D Systems), mouse anti-α-Sma (A5228, Sigma), rabbit anti-Nup98 (2292, Cell Signaling), rabbit anti-Sox2 (2748, Cell Signaling), mouse anti-V5 (46-0705, Life Technologies), mouse anti-Ring1b (39663, Active Motif), rabbit anti-Ring1b (5694S, Cell Signaling), rabbit anti-Rybp (ab5976, Abcam), rabbit anti-Cbx7 (ab21873, Abcam), rabbit anti-Nup50 (ab151567, Abcam), rabbit anti-Suz12 (ab12073, Abcam), rabbit anti-Lmnb2 (ARP46356; Aviva Systems Biology), rabbit anti-Mel-18 (sc-10744, Santa Cruz Biotechnology), and rabbit anti-Foxo1 (2880S, Cell Signaling).
Rabbit anti nup98
Manufactured by Cell Signaling Technology
Rabbit anti-Nup98 is a primary antibody that specifically recognizes the Nup98 protein. Nup98 is a nuclear pore complex protein that plays a crucial role in nucleocytoplasmic transport.
Automatically generated - may contain errors
Lab products found in correlation
Z0334, by Agilent Technologies (1 mentions)
Ab151567, by Abcam (1 mentions)
Rabbit anti parp, by Cell Signaling Technology (1 mentions)
Prb 435p, by BioLegend (1 mentions)
Mouse anti oct3 4, by Santa Cruz Biotechnology (1 mentions)
Mouse anti v5, by Thermo Fisher Scientific (1 mentions)
Rabbit anti foxo1, by Cell Signaling Technology (1 mentions)
Mouse anti gata4, by Santa Cruz Biotechnology (1 mentions)
Ab32423, by Abcam (1 mentions)
Ab12073, by Abcam (1 mentions)
Ab21873, by Abcam (1 mentions)
Mouse anti α tubulin, by Merck Group (1 mentions)
Mouse anti flag, by Merck Group (1 mentions)
Goat anti oct3 4, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti sox2, by Cell Signaling Technology (1 mentions)
Rabbit anti ring1b, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit anti nup98
1
Antibody Characterization for Cell Research
2
Whole Larva Protein Extraction and Western Blot
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To prepare whole larvae lysates, 15 third instar larvae were washed once in PBS 1X with 0.2% Triton X-100 (PBT 0.2%) and once in PBS 1X. Larvae were then put in 200 μl of RIPA lysis buffer (50 mM Tris at pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM PMSF, 1 mM Na3VO4, 10 μg/ml aprotinin, 10 μg/ml leupeptin) and protein were extracted using a pellet pestle adapted for 1.5 ml Eppendorf tube. After 15 min incubation on ice, debris were removed by centrifugation at 12,000g for 15 min at 4°C.
Lysates were resolved on SDS-PAGE and transferred to nitrocellulose membranes. Blotted proteins were immuno-detected using rabbit anti-HOXA9 (1:2000, Milipore Sigma), rabbit anti-NUP98 (1:1000, Cell Signalling), mouse anti-β-Gal (1:2000, Fisher Scientific), and mouse anti-Actin (1:1000, Boehringer Manheim) antibodies.
Lysates were resolved on SDS-PAGE and transferred to nitrocellulose membranes. Blotted proteins were immuno-detected using rabbit anti-HOXA9 (1:2000, Milipore Sigma), rabbit anti-NUP98 (1:1000, Cell Signalling), mouse anti-β-Gal (1:2000, Fisher Scientific), and mouse anti-Actin (1:1000, Boehringer Manheim) antibodies.
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