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Mp 7714

Manufactured by Vector Laboratories
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The MP-7714 is a laboratory instrument designed to perform enzymatic assays. It features a multi-well plate format and a photometric detection system. The core function of the MP-7714 is to measure the absorbance of samples in a 96-well microplate, which is commonly used in various biochemical and cellular analyses.

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Lab products found in correlation

Dfc7000 t camera, by Leica (1 mentions) Application suite x, by Leica (1 mentions) Dm4 microscope, by Leica (1 mentions) Application suite version 2.7.1 r1, by Leica (1 mentions) Bx40 microscope, by Olympus (1 mentions)

2 protocols using mp 7714

1

Double-stained Immunohistochemistry of CXCL12 and CD8

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Slides were deparaffinized by baking overnight at 59 °C, and antigens were retrieved by heating in 0.1% citrate buffer for 10 min at 850 V in a microwave oven. For double-staining immunohistochemistry (IHC), we used ImmPRESS duet staining HRP/AP polymer kits with anti-rabbit IgG-brown and anti-mouse IgG red (MP-7714, Vector Laboratories) according to the manufacturer’s protocol. Nonspecific binding sites were blocked with horse serum. The reactions with anti-CXCL12 (clone, Thermo Fisher) and anti-CD8 were for 16 h at 4 °C. Histology sections were observed using a Leica DM4 microscope. Images were acquired using a Leica DFC7000T camera and Leica Application Suite X.
Zhang L., Cascio S., Mellors J.W., Buckanovich R.J, & Osmanbeyoglu H.U. (2024). Single-cell analysis reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of ovarian cancer. Communications Biology, 7, 20.
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2

Immunohistochemical Analysis of Inflammation Markers

Cited 1 time
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Slides were deparaffinized by baking overnight at 59°C. Endogenous peroxidase activity was eliminated by treatment with 30% H2O2 for 15 min at room temperature. Antigen retrieval was performed by microwave heating in 0.1% citrate buffer for 10 min at 850V. Nonspecific binding sites were blocked with 2% BSA. Reaction with anti-CD163, anti-CD68, anti-CD86, anti-sTn, anti-ST6GALNAC1 (listed above) was for 16h at 4C. Staining was performed by the avidin-biotin-peroxidase complex method with a commercial kit (PK4000, Vectastain ABC HRP kit; Vector Laboratories) according to the manufacturer’s protocol. Positive signals were visualized by a DAB Substrate Kit (cat. #550880, BD Pharmingen) according to the manufacturer’s protocol. The total inflammation score for each sample was determined as previously described [8 (link)]. For double staining IHC, ImmPRESS duet staining HRP/AP polymer kits, anti-rabbit IgG-brown and anti-mouse IgG red was used (MP-7714, Vector Laboratories) according the manufacturer’s protocol. Histology sections were observed using an Olympus BX40 microscope. Images were acquired using a Leica DFC420 camera and Leica Application Suite version 2.7.1 R1.
Kvorjak M., Ahmed Y., Miller M.L., Sriram R., Coronello C., Hashash J.G., Hartman D.J., Telmer C.A., Miskov-Zivanov N., Finn O.J, & Cascio S. (2019). Crosstalk between colon cells and macrophages increases ST6GALNAC1 and MUC1-sTn expression in ulcerative colitis and colitis–associated colon cancer. Cancer immunology research, 8(2), 167-178.
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