Lactate dehydrogenase ldh assay

Manufactured by Abcam

Lactate dehydrogenase (LDH) assay is a colorimetric enzymatic assay used to quantify the activity of the enzyme lactate dehydrogenase in biological samples. The assay measures the conversion of lactate to pyruvate, which is coupled to the reduction of a tetrazolium dye, resulting in a color change that can be detected spectrophotometrically.

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Lab products found in correlation

Cellevent caspase 3 7 green detection reagent, by Thermo Fisher Scientific (3 mentions) Trypan blue, by Merck Group (1 mentions) Fk866, by Merck Group (1 mentions) Stf118804, by Bio-Techne (1 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions) Trypan blue staining, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Lactate dehydrogenase assay (LDH assay) Lactate dehydrogenase (LDH)-cytotoxicity assay kit II Lactate dehydrogenase (LDH) cytotoxicity assay kit Lactate Dehydrogenase (LDH) Assay Kit Lactate dehydrogenase (LDH) Lactate dehydrogenase LDH assay

3 protocols using lactate dehydrogenase ldh assay

Cytotoxicity and Apoptosis Assays

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Cytotoxicity was determined using Trypan blue (Sigma-Aldrich) exclusion assay and lactate Dehydrogenase (LDH) assay (Abcam). Stock solutions for the NAMPT inhibitors STF-118804 (Tocris), CHS 828 (Tocris) and FK 866 (Sigma-Aldrich) were made in DMSO, aliquoted and stored in the freezer until use. Apoptosis was assayed in vitro using CellEvent™ Caspase-3/7 Green Detection Reagent (Thermofisher Scientific) as per manufacturer’s instructions. Experiments were repeated at least 3 times.

Vallejo F.A., Sanchez A., Cuglievan B., Walters W.M., De Angulo G., Vanni S, & Graham R.M. (2022). NAMPT Inhibition Induces Neuroblastoma Cell Death and Blocks Tumor Growth. Frontiers in Oncology, 12, 883318.

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LPS-Induced Cell Death Assessment

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The HUVECs were seeded in a 6-well culture plate one day prior to the experiment and incubated for 24 h. Subsequently, the cells were transferred to 96-well plates. For transfection, the cells were treated with Lipofectamine 2000 reagent (Invitrogen) and transfected with 1 µg/ml of LPS. The transfection was allowed to proceed for 24 h. Cell death was measured using a lactate dehydrogenase (LDH) assay obtained from Abcam, following the instructions provided by the manufacturer.

Yu F., Zhang Z., Leng Y, & Chen A.F. (2023). O-GlcNAc modification of GSDMD attenuates LPS-induced endothelial cells pyroptosis. Inflammation Research, 73(1), 5-17.

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Regulation of NHEK Cytotoxicity

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NHEK was pretreated with AG1478 (1 μM), KN-62 (5 μM), WRW4 (10 μM), zVAD (50 μM) or Nec-1 (50 μM) for 30 min, and then exposed to LL-37 (25 μg/mL) and/or Ac2–26 (50 ng/mL). After 30 h, cytotoxicity was analyzed with trypan blue staining (Gibco) and lactate dehydrogenase (LDH) assay (Abcam).

Kinoshita M., Ogawa Y., Hama N., Ujiie I., Hasegawa A., Nakajima S., Nomura T., Adachi J., Sato T., Koizumi S., Shimada S., Fujita Y., Takahashi H., Mizukawa Y., Tomonaga T., Nagao K., Abe R, & Kawamura T. (2021). Neutrophils initiate and exacerbate Stevens–Johnson syndrome and toxic epidermal necrolysis. Science translational medicine, 13(600), eaax2398.

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