Ab179785

Manufactured by Abcam

Sourced in United States

Ab179785 is a lab equipment product. It is a device used for a specific laboratory function. The core function of this product is to perform a well-defined task in a research or testing environment.

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Lab products found in correlation

Ab49822, by Abcam (2 mentions) Ab44976, by Abcam (2 mentions) Ab140211, by Abcam (2 mentions) Rabbit anti bak, by Cell Signaling Technology (2 mentions) Ab8227, by Abcam (2 mentions) Ab154856, by Abcam (1 mentions) Af6348, by Affinity Biosciences (1 mentions) Recombinant rat il 1β, by Thermo Fisher Scientific (1 mentions) Ab39012, by Abcam (1 mentions) Ab81299, by Abcam (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Ab239007, by Abcam (1 mentions) Rabbit anti bax, by Cell Signaling Technology (1 mentions) Mitochondria isolation kit for tissue, by Beyotime (1 mentions) Rabbit anti irf3 d83b9, by Cell Signaling Technology (1 mentions)

3 protocols using ab179785

Mitochondrial Dysfunction and Apoptosis in Retina

Cited 1 time

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The retinas were collected at 1, 3, 5, and 7 days after the intravitreal injection of mtDNA or control solution. The retinas processed with the Mitochondria Isolation Kit for Tissue as previously described [4 (link)] and the proteins collected from the supernatant and sediment were used for the detection of cytochrome c. Western blotting was performed according to methods described in previous study [4 (link)]. The following primary antibodies were used: rabbit anti-BAX (#2772, Cell Signaling Technology), rabbit anti-BAK (#12105, Cell Signaling Technology), rabbit anti-caspase 9 (AF6348, Affinity Biosciences Ltd), rabbit anti-cleaved caspase 9 (AF5240, affbiotech), rabbit anti-caspase 3 (ab44976, Abcam), rabbit anti-cleaved caspase 3 (ab49822, Abcam), anti-β-actin (ab8227, Abcam), anti-cGAS (ab179785, Abcam), rabbit anti-STING (D1V5L) (#50,494, Cell Signaling Technology), rabbit anti-TBK1/NAK (D1B4) (#3504, Cell Signaling Technology), rabbit anti-phospho-TBK1/NAK (Ser172) (D52C2) XP^® (#5483, Cell Signaling Technology), rabbit anti-IRF3 (D83B9) (#4302, Cell Signaling Technology), rabbit anti-phospho-IRF3 (Ser396) (D6O1M) (#29047, Cell Signaling Technology), anti-interferon β (ab140211, Abcam), rabbit anti-cytochrome c (10993-1-AP, Proteintech), and rabbit anti-VDAC1 (ab154856; Abcam). Three eyes from each group were tested.

Guo Y., Gan D., Hu F., Cheng Y., Yu J., Lei B., Shu Q., Gu R, & Xu G. (2022). Intravitreal injection of mitochondrial DNA induces cell damage and retinal dysfunction in rats. Biological Research, 55, 22.

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Chondrocyte Inflammation and Degradation

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Recombinant rat IL-1β was acquired from Peprotech (RockyHill, NJ, USA). Abcam (Cambridge, MA, USA) provided the antibodies against yH2ax (ab81299), cGAS (ab179785), STING (ab179775), MMP13 (ab39012), ADAMTS5 (ab41037), Collagen II (ab239007), Aggrecan (ab3778), p21 (ab107099) and p16 (ab51243). Moreover, the Cell Signaling Technology (Beverly, MA, USA) provided the antibodies against β-actin (#3700).

Guo Q., Chen X., Chen J., Zheng G., Xie C., Wu H., Miao Z., Lin Y., Wang X., Gao W., Zheng X., Pan Z., Zhou Y., Wu Y, & Zhang X. (2021). STING promotes senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the NF-κB signaling pathway. Cell Death & Disease, 12(1), 13.

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Mitochondrial Dysfunction in Retinal Injury

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The retinas were collected at 1, 3, 5, and 7 days after the intravitreal injection of mtDNA or control solution. The retinas processed with the Mitochondria Isolation Kit for Tissue (C3606, Beyotime, Shanghai, China), as previously described [14] and the proteins collected from the supernatant and sediment were used for the detection of cytochrome c. Western blotting was performed according to methods described in previous study [14] . The following primary antibodies were used: rabbit anti-BAX (#2772, Cell Signaling Technology), rabbit anti-BAK (#12105, Cell Signaling Technology), rabbit anticaspase 9 (AF6348, A nity Biosciences Ltd), rabbit anti-cleaved caspase 9 (AF5240, affbiotech), rabbit anti-caspase 3 (ab44976, Abcam), rabbit anti-cleaved caspase 3 (ab49822, Abcam), anti-β-actin (ab8227, Abcam), anti-cGAS (ab179785, Abcam), rabbit anti-STING (D1V5L) (#50494, Cell Signaling Technology), rabbit anti-TBK1/NAK (D1B4) (#3504, Cell Signaling Technology), rabbit anti-phospho-TBK1/NAK (Ser172) (D52C2) XP® (#5483, Cell Signaling Technology), rabbit anti-IRF3 (D83B9) (#4302, Cell Signaling Technology), rabbit anti-phospho-IRF3 (Ser396) (D6O1M) (#29047, Cell Signaling Technology), anti-interferon β (ab140211, Abcam), rabbit anti-cytochrome c (10993-1-AP, Proteintech), rabbit anti-VDAC1 (ab154856; Abcam). Three eyes from each group were tested.

Guo Y., Gan D., Hu F., Cheng Y., Yu J., Lei B., Shu Q., Gu R., & Xu G. (2021). Intravitreal Injection of Mitochondrial DNA Induces Cell Damage and Retinal Dysfunction in Rats.

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