Biotinylated Sambucus nigra barks lectin was purchased from Vector Laboratory (Burlingame, U.S.A). Phenylmethylsulfonyl fluoride (PMSF) was purchased from Calbiochem (Darmstadt, Germany). Anti-PB1 antibody was prepared by immunization of rabbit with purified PB1 protein. 4′,6-diamino-2-phenylindole (DAPI), rabbit immunoglobulin G (IgG) antibody and mouse IgG antibody with Alexa Flour 488 were purchased from Invitrogen (Carlsbad, CA, U.S.A). Ethylenediamine-N,N,N′,N′-tetraacetic acid (EDTA), N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) and HEPES buffer solution were purchased from Dojindo (Kumamoto, Japan). Blocking one solution and Tris(hydromethyl) amino methane (Tris-base) were purchased from Nacalai tesque (Kyoto, Japan). Acrylamide, N′,N′-methylene bis(acrylamide) (bis), ammonium persulfate (APS), sodium dodecyl sulfate (SDS), paraformaldehyde (PFA) were purchased from Wako Pure Chemical Industries Co., Ltd. (Osaka, Japan). The details of the other reagents used for the nanoprobe fabrication and the cell probing are described in the previous study (Cho et al., 2014 (link)).
N cyclohexyl 3 aminopropanesulfonic acid caps
Manufactured by Dojindo Laboratories
Sourced in Japan
N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) is a zwitterionic organic compound used as a buffering agent in biochemical applications. It maintains a stable pH range and is compatible with a variety of biological systems.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamino 2 phenylindole dapi, by Thermo Fisher Scientific (1 mentions)
Ethylenediamine n n n n tetraacetic acid edta, by Dojindo Laboratories (1 mentions)
Proline, by Fujifilm (1 mentions)
Tyrosine, by Fujifilm (1 mentions)
Histidine, by Fujifilm (1 mentions)
Phenylalanine, by Fujifilm (1 mentions)
Tryptophan, by Fujifilm (1 mentions)
1 4 7 triazacyclononane 1 4 7 triacetic acid nota, by Macrocyclics (1 mentions)
2 protocols using n cyclohexyl 3 aminopropanesulfonic acid caps
1
Sambucus nigra Lectin-based Nanoprobe Protocol
2
Lanthanide-Based Affinity Ligand Synthesis
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All reagents employed were of analytical grade. Neu5Ac and disialic acid (diNeu5Ac) were purchased from Nagara Science (Gifu, Japan). The affinity ligands, S-2-(4-aminobenzyl)-1,4,7triazacyclononane-1,4,7-triacetic acid (ABNOTA) and 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) were purchased from Macrocyclics (Dallas, TX). Chloride salts of lanthanides (lanthanum, europium, terbium, dysprosium, erbium, ytterbium and lutetium) were obtained from Sigma-Aldrich (St. Louis, MO). The pH buffer reagent, N-cyclohexyl-3-aminopropanesulfonic acid (CAPS), was employed (Dojindo, Kumamoto, Japan). Dimethyl sulfoxide (DMSO), glycine, proline, phenylalanine, tryptophan, histidine and tyrosine were purchased from Wako Pure Chemical Industry (Osaka, Japan). D-fructose, D-glucose and D-ribose were purchased from Kanto Kagaku (Tokyo, Japan). All stock solutions were prepared by dissolving appropriate amounts of the solid reagents into ultrapure water (>18.2 MΩ•cm), produced by Direct-Q 3UV (Nihon Millipore, Tokyo, Japan).
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