Mouse anti-iNOS (ab49999), Rabbit anti-Mannose Receptor (ab64693), Mouse anti-Osteocalcin (ab13420), Rabbit anti-Alkaline Phosphatase (ab218574) were obtained from abcam, USA; DyLight 488, Goat Anti-Mouse IgG (A23210, Abbkine, USA); DyLight 594, Goat Anti-Rabbit IgG (A23420, Abbkine, USA); Phalloidine (40735ES75, YEASEN, China); DAPI (C1005, Beyotime, China); M-CSF (400-28, Peprotech, USA); IL-4 (400-04, Peprotech, USA).
Phalloidine
Manufactured by Yeasen
Sourced in United States, China
Phalloidine is a chemical compound used in biological research laboratories. It serves as a selective and high-affinity probe for filamentous actin (F-actin), a structural component of the cytoskeleton in eukaryotic cells. Phalloidine is commonly utilized in immunofluorescence and microscopy techniques to visualize and study the organization and dynamics of the actin cytoskeleton.
Automatically generated - may contain errors
Lab products found in correlation
Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igg, by Abbkine (1 mentions)
Dylight 594, by Abbkine (1 mentions)
Dylight 488, by Abbkine (1 mentions)
M csf, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Goat anti rabbit igg, by Abbkine (1 mentions)
Insulin, by Wuhan Servicebio Technology (1 mentions)
Second antibody, by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Beyotime (1 mentions)
Confocal microscopy, by Olympus (1 mentions)
2 protocols using phalloidine
1
Characterization of Osteoclast Differentiation
2
Immunofluorescence Staining of Pancreatic Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For pancreatic sections, mouse pancreases were collected, fixed in 4% paraformaldehyde, embedded in paraffin and cut into sections (5 μm) for further stanning overnight at 4°C with primary antibodies: β-catenin (CST, Cat#8480, 1:200), insulin (Servicebio, GB11334-100, 1:1000), and PDX1 (Abcam, ab219207, 1:1000). On the second day, sections were washed in PBS containing with 1% tween 20 (PBST), and then incubated with second antibody (ThermoFisher, 1:500) at 37°C for 1h. Then sections were washed in PBST and stained with Hoechst 33342 (Beyotime, 1:8000) before imaging by confocal microscopy (Olympus). For cell lines, Min6 cells were stained with phalloidine (Yeasen, Shanghai, China) according to the manufacturer’s protocol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!