Mouse anti nf κb p65 antibody

Fifty milligrams of ischemic penumbra tissues (n = 6) was used for Western blot analysis as previously described [15 (link)]. The primary antibodies include the following: mouse anti-VE-cadherin (1:1000, Cell Signaling Technology), rabbit anti-AQP4 (1:1000, Proteintech, USA), rabbit anti-Claudin-1 (1:1000, Abcam, USA), mouse anti-GFAP (1:1000, Cell Signaling Technology), rabbit anti-Iba1 antibody (1:500, Wako), goat anti-C3d antibody (1:1000, RD Systems), rabbit anti-S100A10 (1:1000; Invitrogen), rabbit anti-TLR4 antibody (1:1000, Cell Signaling Technology), mouse anti-phospho-NF-κB p65 (Ser536) antibody (1:1000, Abcam), mouse anti-NF-κB p65 antibody (1:1000, Abcam), mouse anti-GAPDH (1:1000, Cell Signaling Technology). The membranes were then incubated with the corresponding HRP-conjugated secondary antibody for 2 h. Protein bands were visualized using the LI-COR Odyssey System (LI-COR Biotechnology, USA).

Rabbit anti-phosphorylated and anti-total p38MAPK mAb, rabbit anti-phosphorylated and anti-total IκB mAb, rabbit anti-phosphorylated and anti-total AKT mAb, rabbit anti-Mcl-1 mAb, rabbit anti-procaspase 3 mAb, mouse anti-active caspase-3 mAb, mouse anti-β-actin mAb, rabbit anti-IgG, and mouse anti-IgG were purchased from Cell Signaling Technology (Beverly, MA, USA). Mouse anti-His mAb was purchased from Affinity Biosciences, Inc (OH, USA). The rabbit anti-C. psittaci mAb was a gift from Professor G. Zhong (University of Texas Health Science Center at San Antonio, TX). The rabbit anti-SOD2 antibody, mouse anti-Bax antibody was purchased from ImmunoWay Biotechnology Company (Plano, TX, USA). The inhibitors SB203580, BAY117082, LY294002, staurosporine (STS), and Cy2-conjugated goat anti-rabbit IgG, DAPI, Cy3-conjugated goat anti-mouse IgG, and Cy3-conjugated goat anti-rabbit IgG and mouse anti-NF-κB p65 antibody were obtained from Abcam (Cambridge, MA, USA), recombinant human IL-8 from (Biolegend, CA, USA), anti-IL-8 mAb was obtained from GeneTex, USA.