The ATR inhibitor VE822 and the CDK1 inhibitor (RO-3306) were purchased from Selleck (S7102) and Calbiochem (217699) respectively. Hydroxyurea (HU) was purchased from TCI Chemicals (H0310), Cordycepin and N-Acetyl-cysteine (NAC) were purchased from Wako (017-05131), 5,6-dichloro-1-β-dribofuranosylbenzimidazole (DRB) from Cayman Chemical (10010302), and triptolide from AdipoGen (AG-CN2-0448). 5-Ethynyl Uridine (EU) was purchased from Thermo Fisher Scientific (E10345). CDC7 inhibitor PHA-767491 was purchased from Sigma-Aldrich (PZ0178). Concentrations and conditions used in each assay are listed in Figure legends.
Triptolide
Manufactured by Adipogen
Triptolide is a natural compound extracted from the thunder god vine (Tripterygium wilfordii). It is a potent small molecule with immunosuppressive and anti-inflammatory properties. Triptolide has been widely used as a research tool for studying various biological processes in cell-based and animal studies.
Automatically generated - may contain errors
Lab products found in correlation
Cordycepin, by Fujifilm (1 mentions)
N acetylcysteine, by Fujifilm (1 mentions)
5 6 dichloro 1 β d ribofuranosylbenzimidazole drb, by Cayman Chemical (1 mentions)
Ro 3306, by Selleck Chemicals (1 mentions)
Ve 822, by Selleck Chemicals (1 mentions)
Pha 767491, by Merck Group (1 mentions)
5 ethynyl uridine, by Thermo Fisher Scientific (1 mentions)
Formaldehyde, by Nacalai Tesque (1 mentions)
Cb 5083, by Selleck Chemicals (1 mentions)
Nms 873, by Merck Group (1 mentions)
293ft cell, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions)
2 protocols using triptolide
1
Small Molecule Inhibitor Protocol
2
Cell Culture and Genetic Manipulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HeLa cells (laboratory stock) and 293FT cells (Thermo Fisher Scientific) were maintained in Dulbecco’s modified Eagle medium (DMEM, FUJIFILM) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and antibiotics, unless otherwise noted. Mycoplasma testing was performed routinely. Cell lines stably expressing Strep-V5-GFP-TFIIS were obtained by selecting HeLa cells transfected with pEGFP-C1-Strep-V5-GFP-TFIIS plasmid in medium containing 500 μg ml−1 G418 (Nacalai Tesque). HeLa ΔCSB cells were transfected with pEGFP-C1-Strep-V5-GFP-CSB plasmid in medium containing 500 μg ml−1 G418 (Nacalai Tesque). Unless otherwise indicated, the following doses of chemicals were used: CB5083 (10 μM, Selleck), DRB (100 μM, TCI), epoxomicin (1 μM, FUJIFILM), formaldehyde (600 μM, Nacalai Tesque), MG262 (1 μM, AdipoGen Life Sciences), NMS873 (10 μM, Sigma-Aldrich) and triptolide (3 μM, AdipoGen Life Sciences).
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