Plasmid pDsRed-MAX-N1 was purchased from Addgene (Watertown, MA, USA). DH5–α competent E. coli was purchased from New England Biolabs Inc (Ipswich, MA, USA). Plasmid Giga Kit was purchased from Qiagen (Germantown, MD, USA). Chitosan was purchased from Acmey Industrial (Shanghai, China). DAPI, Lipofectamine 3000, 2-Iminothiolane (Traut’s reagent), Succinimidyl Iodoacetate (SIA), Ultrapure Agarose, antibiotic-antimycotic, Tryple Express Enzyme solution, RPMI 1640 and DMEM cell culture medium were purchased from Invitrogen (Carlsbad, CA, USA). PD10 desalting columns, Sephacryl S-200 resin, and HyClone characterized fetal bovine serum (FBS) were purchased from GE Healthcare Life Sciences (Pittsburgh, PA, USA). NHS-Cy5 and NHS-AF488 were purchased from Lumiprobe Corp (Hunt Valley, MD, USA). Label IT Tracker Intracellular Nucleic Acid Localization Kits were purchased from Mirus Bio (Madison, WI, USA). SpectraPOR7 dialysis membrane was purchased from Repligen Corp (Waltham, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St Louis, MO, USA).
Hyclone characterized fetal bovine serum
Manufactured by GE Healthcare
Sourced in United States
HyClone characterized fetal bovine serum (FBS) is a laboratory reagent used in cell culture applications. It provides essential growth factors, hormones, and other nutrients required for the proliferation and maintenance of a variety of cell lines. The product is rigorously tested and characterized to ensure consistent performance and quality.
Automatically generated - may contain errors
Lab products found in correlation
Ultrapure agarose, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Cy5 nhs, by Lumiprobe (2 mentions)
Sephacryl s 200 resin, by GE Healthcare (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Tryple express enzyme solution, by Thermo Fisher Scientific (2 mentions)
Pd 10 desalting column, by GE Healthcare (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Cell culture medium, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Dh5α competent e coli, by New England Biolabs (1 mentions)
Plasmid giga kit, by Qiagen (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Collagenase type 2, by Worthington (1 mentions)
Cell strainer, by Corning (1 mentions)
Dispase, by Thermo Fisher Scientific (1 mentions)
Annexin 5 alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Tris glycine buffer, by Bio-Rad (1 mentions)
Precision plus protein kaleidoscope prestained protein standard, by Bio-Rad (1 mentions)
Laemmli sample buffer, by Bio-Rad (1 mentions)
4 protocols using hyclone characterized fetal bovine serum
1
Nanoparticle-mediated Plasmid Delivery
2
Dissociation of Gonadal Adipose Tissue
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A single-cell suspension was obtained using the tissue dissociation methods described previously [30 (link),31 (link),32 (link),40 (link)], with slight modifications. The GA was minced with scissors in a 35 mm dish containing ~100 μL dissociation media consisting of Dulbecco’s Modified Eagle Medium (DMEM; Thermo Fisher; Waltham, MA, USA), 600–700 U/mL Collagenase Type II (Worthington Biochemical; Lakewood, NJ, USA), and 0.5 U/mL Dispase (Thermo Fisher) for 3 min and placed on ice. Minced tissue was then incubated in 10 mL dissociation media at 37 °C with gentle agitation for 1 h. Enzymatic digestion was quenched via the addition of growth media (DMEM containing 20% HyClone characterized fetal bovine serum (FBS; GE Healthcare, Lot #A00168; Chicago, IL, USA) followed by serial filtration of the cell suspension through 100 µm and 70 µm cell strainers (Corning Life Sciences; Tewksbury, MA, USA).
3
Preparation of Doxorubicin and Paclitaxel Nanoparticles
4
Chitosan-based Nanoparticles for mRNA Delivery
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All chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated. Chitosan (2.3 kMW) was obtained from Acmey Industrial (Shanghai, China). Branched PEI (MW 2 kDa) was purchased from Polysciences (Warrington, PA, USA). CleanCap EGFP mRNA was purchased from TriLink Biotechnologies (San Diego, CA, USA). Low molecular weight heparin used in PPH polymer was purchased from Galen Laboratory Supplies (North Haven, CT, USA). Furthermore, (1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride) (EDC), N-hydroxysuccinimide (NHS), Lab-Tek II 8-well chambered coverglass, NucBlue DAPI reagent, Lipofectamine 2000, LysoTracker Red DND-99, ultrapure agarose, antibiotic-antimycotic (100×), Tryple Express Enzyme solution, RPMI 1640, and DMEM cell culture medium were purchased from Invitrogen (Carlsbad, CA, USA). HyClone characterized fetal bovine serum (FBS) was purchased from GE Healthcare Life Sciences (Pittsburgh, PA, USA). Label IT Tracker Intracellular Nucleic Acid Labeling Kits were purchased from Mirus Bio (Madison, WI, USA). Cyanine5 NHS ester was purchased from Lumiprobe Corporation (Cockeysville, MD, USA). SpectraPOR7 dialysis membrane was purchased from Repligen Corp (Waltham, MA, USA). Moreover, 4T1, HepG2 and C6 cell lines were purchased from American Type Culture Collection (Manassas, VA, USA).
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