The human GBM cell lines U-138MG (U-138 MG ATCC® HTB-16™ Homo sapiens brain glioblastoma) and U-87 MG (U-87 MG ATCC® HTB-14™ Homo sapiens brain Likely glioblastomas) were obtained from the ATCC (American Type Culture Collection, Rockville, MD, USA). Cell lines U-138 and U-87 were derived from human malignant gliomas and are commonly used as experimental models of glioblastoma13,14 (link). U-138 differs from U-87 in morphology, invasion, and proliferation rate13 (link). U-138 and U-87 exhibit different protein expression profiles, mainly characterized by a very low expression of glial fibrillary acidic protein (GFAP), generally expressed in glial cells and almost completely absent in U-87 cells15,16 (link). GBM cells lines were cultured in a 75-cm2 flask with, respectively, ATCC-formulated Eagle’s Minimum Essential Medium (Catalog No. 30-2003; ATCC) for U-138 MG and Dulbecco’s modified Eagle’s medium (DMEM; Catalog No. D5030; Sigma-Aldrich, St. Louis, MO, USA) for U-87 MG, both supplemented with antibiotics (penicillin, 1000 U; streptomycin, 0.1 mg/L; Catalog No. P4333; Sigma-Aldrich), l -glutamine (GlutaMAX™, Catalog No. 35050061; ThermoFisher Scientific, Waltham, MA, USA), and 10% (v/v) fetal bovine serum (FBS; Catalog No. 12103C; Sigma-Aldrich) in a humidified atmosphere containing 5% CO2 at 37° C.
Catalog no 30 2003
Manufactured by American Type Culture Collection
Sourced in United States
Catalog No. 30-2003 is a laboratory equipment item from the American Type Culture Collection. It serves as a core function, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Catalog no d5030, by Merck Group (2 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
U138mg, by American Type Culture Collection (1 mentions)
U 87 mg atcc htb 14 homo sapiens brain, by American Type Culture Collection (1 mentions)
U87mg, by American Type Culture Collection (1 mentions)
2 protocols using catalog no 30 2003
1
Culturing Human Glioblastoma Cell Lines
2
Glioblastoma Cell Line Cultivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human GB cell lines: U87 MG (U87 MG ATCC® HTB-14™ Homo sapiens brain likely glioblastomas), U138MG (U138 MG ATCC® HTB-16™ Homo sapiens brain glioblastoma IV grade), and A172 (A172\ATCC® CRL-1620™ Homo sapiens brain glioblastoma) were obtained from ATCC (American Type Culture Collection, Rockville, MD, USA). The GB human cell lines U87, U138 and A172 were cultured in 75 cm2 flask with, respectively, ATCC-formulated Eagle’s Minimum Essential Medium (Catalog No. 30-2003; ATCC, Rockville, MD, USA) for U138 and Dulbecco’s modified Eagle’s medium (DMEM—Sigma-Aldrich® Catalog No. D5030; St. Louis, MO, USA) for U87 and A-172, both supplemented with antibiotics (Penicillin 1000 units—Streptomycin 0.1 mg/L, Sigma-Aldrich® Catalog No. P4333; St. Louis, MO, USA), L-glutamine (GlutaMAX™, ThermoFisher Scientific® Catalog No. 35050061; Waltham, MA, USA) and 10% (v/v) Fetal bovine serum (FBS, Sigma-Aldrich® Catalog No. 12103C St. Louis, MO, USA). Normal human astrocytes (NHA) (Lonza, Walkersville, MD, USA) were used as normal counterparts [55 (link),56 (link)]; NHA cells were grown in astrocyte medium-containing supplements (Allcells) with 10% FBS. All cell lines are maintained in the incubator with a humidified atmosphere containing 5% CO2 at 37 °C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!