Pa5 29183

Tubular injury was semiquantitatively assessed in PAS-stained kidney tissues. To detect monocytes/macrophages or neutrophils, formalin-fixed, paraffin-embedded kidney tissues were stained with monoclonal antibodies against F4/80 (1:100; Bio-Rad Laboratories, Hercules, CA, USA) and Ly6G (1:200; eBioscience, San Diego, CA, USA). The mean number of positive cells per 8–10 high-power fields (HPFs) was compared. Tubular cell apoptosis was quantified by counting the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive epithelial cells in 8–10 HPFs (200×). NGAL (1:1000; Abcam, Cambridge, UK), anti-8-OHdG antibody [N45.1] (ab48508; ABcam, Cambridge, UK), SOD (1:800; MA1-105, ThermoFisher, Berkeley, MO, USA), catalase (1:400, PA5-29183, ThermoFisher, Waltham, MA, USA), KIM-1 (1:200, MBS2006453, MyBioSource, San Diego, CA, USA), and IGFBP7 (1:1000; Abcam, Cambridge, UK)-positive cells were semiquantified using the ImageJ software (National Institutes of Health, Bethesda, MD, USA) [34 (link)].