Wild-type C57BL/6 mice (male, 6-weeks old) were purchased from SPF Biotechnology Ltd (Beijing, China). All the animal experiments were approved by the Ethical Committee of Huazhong University of Science and Technology and performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. The mice were housed in a specific pathogen-free room with a controlled light/dark cycle (lights on 08:00–20:00).
Recombinant adeno-associated virus (AAV) vectors of serotype 9 harboring the murine PRMT4 gene were generated by replacing the mCherry fragment in the AAV cloning vector pAOV-CMV-mCherry with PRMT4-3X Flag.28 (link) The vector pAOV-CMV-mCherry was used to produce empty vectors for transduction into control mice.
T1DM was induced in 6 weeks old male mice by injection of STZ dissolved in 100 mmol/L citrate buffer as described previously29 (link) with some modifications. Briefly, mice were fasted overnight and then injected intraperitoneally with a dose of STZ (150 mg/kg). One week after intraperitoneal injection, mice were monitored for hyperglycemia on days 7, 10, and 14. Only mice in which random blood glucose exceeded 16.7 mM for the following week were assigned to the diabetic group.
Recombinant adeno-associated virus (AAV) vectors of serotype 9 harboring the murine PRMT4 gene were generated by replacing the mCherry fragment in the AAV cloning vector pAOV-CMV-mCherry with PRMT4-3X Flag.28 (link) The vector pAOV-CMV-mCherry was used to produce empty vectors for transduction into control mice.
T1DM was induced in 6 weeks old male mice by injection of STZ dissolved in 100 mmol/L citrate buffer as described previously29 (link) with some modifications. Briefly, mice were fasted overnight and then injected intraperitoneally with a dose of STZ (150 mg/kg). One week after intraperitoneal injection, mice were monitored for hyperglycemia on days 7, 10, and 14. Only mice in which random blood glucose exceeded 16.7 mM for the following week were assigned to the diabetic group.