Nlrp3

Manufactured by Gentex

NLRP3 is a laboratory product developed by Gentex. It is a protein complex involved in the inflammatory response. The core function of NLRP3 is to act as a sensor for detecting cellular stress and damage, which can trigger the activation of the inflammasome, a multi-protein complex that initiates the inflammatory response.

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Lab products found in correlation

Cd 11b, by Gentex (2 mentions) Tunel kit, by Thermo Fisher Scientific (2 mentions) Pla goat plus, by Merck Group (2 mentions) Duolink pla rabbit minus, by Merck Group (2 mentions) Duolink pla kit, by Merck Group (2 mentions) Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Polyvinylidene difluoride pvdf membrane, by Merck Group (1 mentions) Cleaved caspase 1, by Cell Signaling Technology (1 mentions) P creb, by Cell Signaling Technology (1 mentions) Cleaved il 1β, by Cell Signaling Technology (1 mentions) Polyvinylidene di uoride pvdf membranes, by Merck Group (1 mentions) 5 bromo 4 chloro 3 indolyl phosphate bcip, by Promega (1 mentions) Bcl 2, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions)

2 protocols using nlrp3

Antibody-based Protein Analysis Protocol

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The following antibodies were used: NeuN (Millipore Biotechnology, MAB377); GPER (sc-48525); Iba1 (sc-32725), GAPDH (sc-32233), NLRP3 (Life science, Lot L27693), NLRP3 (ab4207), ASC (sc-22514-R), CD 11b (Gentex, GTX76060), cleaved caspase 1 (cell signaling, #4199S), cleaved IL1β (sc-7884), IL1RA (ab124962), and IL1RA blocking peptide (ab200257); and NF-κB-p65 (ab32568), H2A (ab177308), Bcl2 (sc-492), cleaved caspase 3 (D175, 5A1E), CREB (cell signaling, #9197X), and p-CREB (cell signaling, #9198S), tubulin (sc-9104). Alexa-conjugated secondary antibodies were from Molecular Probes/Invitrogen (Carlsbad, CA). Polyvinylidene difluoride (PVDF) membranes with pore size of 0.45 μm were from Millipore (USA). BCIP (5-bromo-4-chloro-3-indolyl-phosphate) and NBT (nitro blue tetrazolium) were from Promega (Madison, WI). TUNEL Kits (LOT #1639496, Life technologies) were from Active Motif Company. Duolink PLA kits (DUO92105), Duolink PLA Rabbit MINUS (DUO92003), and PLA Goat PLUS (DUO92005) proximity probes were from Sigma-Aldrich. Unless indicated otherwise, all the other chemicals were from Sigma-Aldrich (St. Louis, MO).

Bai N., Zhang Q., Zhang W., Liu B., Yang F., Brann D, & Wang R. (2020). G-protein-coupled estrogen receptor activation upregulates interleukin-1 receptor antagonist in the hippocampus after global cerebral ischemia: implications for neuronal self-defense. Journal of Neuroinflammation, 17, 45.

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Comprehensive Immunohistochemistry and Western Blot

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The following antibodies were used: NeuN (Millipore Biotechnology, MAB377); GPER (sc-48525); Iba1 (sc-32725), GAPDH (sc-32233), NLRP3 (Life science, Lot L27693), NLRP3 (ab4207), ASC (sc-22514-R), CD 11b (Gentex, GTX76060 ), Cleaved caspase 1 (Cell signaling, #4199S ), Cleaved IL1β (sc-7884), IL1RA (ab124962), and IL1RA blocking peptide (ab200257), NFkB-p65 (ab32568), H2A (ab177308), Bcl2 (sc-492), Cleaved caspase 3 (D175, 5A1E), CREB (Cell signaling, #9197X), p-CREB (Cell signaling, #9198S), Tubulin (sc-9104). Alexa-conjugated secondary antibodies were from Molecular Probes/Invitrogen (Carlsbad, CA). Polyvinylidene di uoride (PVDF) membranes with pore size of 0.45 µm were from Millipore (USA). BCIP (5-bromo-4-chloro-3-indolyl-phosphate) and NBT (nitro blue tetrazolium) were from Promega (Madison, WI). TUNEL Kits (LOT #1639496, Life technologies) were from Active Motif Company. Duolink PLA kits (DUO92105), Duolink PLA Rabbit MINUS (DUO92003) and PLA Goat PLUS (DUO92005) proximity probes were from SIGMA-ALDRICH. Unless indicated otherwise, all the other chemicals were from Sigma-Aldrich (St. Louis, MO).

Bai N., Zhang Q., ZHANG W., Liu B., Liu S., Brann D.W., & Wang R. (2020). G-Protein-Coupled Estrogen Receptor Activation Upregulates Interleukin-1 Receptor Antagonist in the Hippocampus after Global Cerebral Ischemia: Implications for Neuronal Self Defense.

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