Sars cov 2 2019 ncov spike s1 s2 ecd his recombinant protein

The SARS-CoV-2 (2019-nCoV) Spike S1-His recombinant protein containing SARS-CoV-2 spike protein S1 region (S1, Val16-Arg685), the SARS-CoV-2 (2019-nCoV) Spike S1 + S2 ECD-His recombinant protein containing the ectodomain of the S1 and S2 region (S1 + S2 ECD, Val16-Pro1213), and podoplanin (PDPN) were purchased from Sino Biological (Wayne, PA, USA). The high molecular weight polyinosinic-polycytidylic acid (poly(I:C)) was purchased from InvivoGen (San Diego, CA, USA). Collagen was purchased from Chrono-log (Havertown, PA, USA). Thrombin was purchased from Calbiochem (Darmstadt, Germany). U46619 was purchased from Cayman Chemical (Ann Arbor, MI, USA). Phorbol 12-myristate 13-acetate (PMA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Corning BioCoat 12 mm No. 1 German Glass Coverslips and the SYTOX Green nucleic acid stain reagent were purchased from ThermoFisher (Waltham, MA, USA).