Soil pH (H 2 O) was determined via Lab pH meter (inoLab ® pH 7110-WTW, by Xylem Analytics -Weilheim in Oberbayern, Germany) upon stirring a mixture of soil and deionized water according to ISO 10390 standard. Soil organic carbon was measured by a TOC analyzer (SKALAR PrimacsSLC). Soil bulk densities were determined at each depth by collecting the soil in standard-size steel cylinders (113 cm 3 ) and weighing the sample before and after oven drying for 48 h at 105 • C.
Phospholipid fatty acids (PLFA) analysis was performed to determine the soil microbial biomass and microbial community structure according to the ISO/TS 29843-2. PLFAs extraction from soil samples is described by Holík et al. [29] . Briefly, soil was extracted by mixture of chlorophorm, methanol and phosphate buffer. The polar lipid fraction was separated on silica columns and fatty acid methyl esters (FAMEs) were prepared by alkaline methanolysis. PLFAs were quantitatively analyzed by Varian 3800 gas chromatograph with DB-FFAP capillary column (60 m × 0.25 mm × 0.50 µm, Agilent); helium as carrier gas (constant flow 0.8 mL/min); splitless injection (1 µL, 0.
Phospholipid fatty acids (PLFA) analysis was performed to determine the soil microbial biomass and microbial community structure according to the ISO/TS 29843-2. PLFAs extraction from soil samples is described by Holík et al. [29] . Briefly, soil was extracted by mixture of chlorophorm, methanol and phosphate buffer. The polar lipid fraction was separated on silica columns and fatty acid methyl esters (FAMEs) were prepared by alkaline methanolysis. PLFAs were quantitatively analyzed by Varian 3800 gas chromatograph with DB-FFAP capillary column (60 m × 0.25 mm × 0.50 µm, Agilent); helium as carrier gas (constant flow 0.8 mL/min); splitless injection (1 µL, 0.