Ethyl alcohol was utilized for depara nization and re-hydration of prostate sections. This was followed by a 10 min boiling of the sections in citrate buffer (pH 6.0). Then, sections were kept in 5% bovine serum albumin in tris buffered saline (BSA/TBS) for 2 hours. Primary antibodies, anti-cyclin D1 (ab16663), anti-IL-6 (ab271269) and anti-TNF-α (ab205587) (ABCAM, Cambridge, UK) were used for incubation at 4 o C for 12 h. The slides were washed using TBS and further incubated with a biotinylated secondary antibody (Ant-rabbit HPRD-DAB, Catalog # CTS005, R&D systems, MN, USA). Images were analyzed and quanti ed from at least three rats using Image J software (Image J, 1.46a, NIH, USA).
Ab271269
Manufactured by Abcam
Sourced in United Kingdom
Ab271269 is a product offered by Abcam. It is a piece of lab equipment, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab271269
1
Immunohistochemical Analysis of Prostate Biomarkers
2
Immunohistochemical Analysis of Prostate Biomarkers
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Ethyl alcohol was utilized for depara nization and re-hydration of prostate sections. This was followed by a 10 min boiling of the sections in citrate buffer (pH 6.0). Then, sections were kept in 5% bovine serum albumin in tris buffered saline (BSA/TBS) for 2 hours. Primary antibodies, anti-cyclin D1 (ab16663), anti-IL-6 (ab271269) and anti-TNF-α (ab205587) (ABCAM, Cambridge, UK) were used for incubation at 4 o C for 12 h. The slides were washed using TBS and further incubated with a biotinylated secondary antibody (Ant-rabbit HPRD-DAB, Catalog # CTS005, R&D systems, MN, USA). Images were analyzed and quanti ed from at least three rats using Image J software (Image J, 1.46a, NIH, USA).
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