For western blot analyses, adult fly heads were homogenized in 2× Laemmli sample buffer, and the lysates were separated by SDS-PAGE. Membranes were blocked with 5% non-fat dry milk and probed with anti-Sra (1:1000; a gift from Dr Toshiro Aigaki), anti-Aβ42 (1:2000; 6E10, Covance, UK), anti-Myc (1:2000; Cell Signaling Technology, Beverly, MA, USA) or anti-Actin (1:2000; JLA20, DSHB, Iowa City, IA, USA) antibodies. Western blot analyses were performed using standard procedures and horseradish-peroxidase-conjugated secondary antibodies (1:2000; Cell Signaling Technology, Beverly, MA, USA).
Anti aβ42
Manufactured by Fortrea
Sourced in United Kingdom
Anti-Aβ42 is a laboratory product designed to detect the presence of the amyloid-beta 42 (Aβ42) peptide. Aβ42 is a key component of amyloid plaques, which are associated with Alzheimer's disease. The primary function of Anti-Aβ42 is to provide a tool for researchers to analyze and quantify Aβ42 levels in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti myc, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Anti atg7, by Cell Signaling Technology (1 mentions)
Anti beclin1, by Abcam (1 mentions)
Anti atg12, by Cell Signaling Technology (1 mentions)
α syn, by Thermo Fisher Scientific (1 mentions)
Anti lc3b, by Cell Signaling Technology (1 mentions)
Anti actin, by Thermo Fisher Scientific (1 mentions)
Anti 6e10, by Fortrea (1 mentions)
2 protocols using anti aβ42
1
Western Blot Analysis of Fly Brains
2
Immunoblotting of Soluble Protein Fractions
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Soluble fractions were probed with (1:1000) mouse monoclonal anti-6E10 (Covance SIG-39320), (1:1000) rabbit polyclonal anti-Aβ42 (Covance SIG-39153), (1:1000) rabbit monoclonal anti-AT180 (Cat. #151559, Abcam, Cambridge, Cambridgeshire, England), (1:1000) rabbit polyclonal anti-Beclin-1 (Cat. #D40C5, Cell Signaling Technology, Danvers, Massachusetts, USA), (1:1000) rabbit polyclonal anti-Atg7 (Cat. #D12B11, Cell Signaling Technology), (1:1000) rabbit polyclonal anti-Atg12 (Cat. #D88H11, Cell Signaling Technology), (1:1000) rabbit polyclonal anti-LC3B (Cat. #PA1-16931, Invitrogen Inc.), α-syn (Cat. #Ma1-12874, ThermoFisher, Waltham, Massachusetts, USA), and (1:1000) rabbit polyclonal anti-actin (Cat. #MAB1501R, Millipore, Burlington, Massachusetts, USA).
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