The initial natural pH of the milk samples and the pH of the liquid chyme (or emptied liquid chyme) samples from the HGS at each time point were measured using a CyberScan pH 510 pH/mV/°C meter (Eutech Instruments, Fisher, Malaysia). The pH of the coagulated phase was also measured, by inserting the pH probe inside the clots (close to the center). All referenced pH values correspond to the pH of the liquid chyme, unless specified otherwise.
Cyberscan ph 510
Manufactured by Thermo Fisher Scientific
Sourced in United States, India, Malaysia, Singapore
The CyberScan pH 510 is a pH meter designed for accurate pH measurement in laboratory settings. It features a digital display for easy readability and supports temperature compensation for precise pH readings.
Automatically generated - may contain errors
20 protocols using cyberscan ph 510
1
pH Measurement of Milk and Chyme
2
Meat Homogenate pH Measurement
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The pH of the meat homogenates were determined with a glass electrode pH meter
(Cyberscan pH 510, Eutech Instruments, Vernon Hills, IL, USA). The pH meter was
calibrated using pH 7.0 and 4.0 standard buffers stored at room temperature.
Meat homogenate was prepared by blending finely-chopped meat with milli-Q water
in a ratio of 1:10 for 1 min using a food processor (BFP100WHT, Breville,
Sydney, Australia).
(Cyberscan pH 510, Eutech Instruments, Vernon Hills, IL, USA). The pH meter was
calibrated using pH 7.0 and 4.0 standard buffers stored at room temperature.
Meat homogenate was prepared by blending finely-chopped meat with milli-Q water
in a ratio of 1:10 for 1 min using a food processor (BFP100WHT, Breville,
Sydney, Australia).
3
Measuring Honey pH and Acidity
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The harmonized methods
of the International Honey Commission were used to obtain the pH and
free acidity.18 (link) A total of 10 g of the
honey samples were dissolved in 75 mL of CO2-free distilled
water, and the pH of the solution was measured using a pH meter (CyberScan
pH510—Eutech Instruments). The honey CO2-free distilled
water solution was titrated using 0.1 M NaOH to pH 8.3 to measure
the free acids. The results were expressed in milliequivalents per
kilogram. To obtain the mean value, three replicates were created
and used.
of the International Honey Commission were used to obtain the pH and
free acidity.18 (link) A total of 10 g of the
honey samples were dissolved in 75 mL of CO2-free distilled
water, and the pH of the solution was measured using a pH meter (CyberScan
pH510—Eutech Instruments). The honey CO2-free distilled
water solution was titrated using 0.1 M NaOH to pH 8.3 to measure
the free acids. The results were expressed in milliequivalents per
kilogram. To obtain the mean value, three replicates were created
and used.
4
Fish Muscle pH Measurement
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After homogenization of a suitable amount of fish muscle, the pH was measured using Cyber Scan pH-510 digital pH-meter (EUTECH Instruments, Breda, The Netherlands).
5
Determining pKa of ABT-737 by Titration
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Titration curves of ABT-737 were generated based on pH measurements performed at room temperature (CyberScan pH 510, Eutech Instruments, Thermo Scientific, Walthman, MA), versus added volume, V, of 0.01 M HCl. The first derivative of the titration curves, versus the titrant volume, dpH/dV, were utilized to determine the pKa and equivalence values of the drug's ionizable groups. The titrated ABT-737 solution, 0.15 mg/mL, 0.5 mL, was prepared by diluting ABT-737 in DMSO solution (0.5 mg/mL, 0.61 mM) in DIW (30:70% v/v). This ABT-737 solution was supplemented with 5 μL of 0.1 M NaOH to raise the pH to 9.5. The titration was conducted with 10 mL aliquots of the HCl solutions, accompanied by measurement of the pH, up to a total added volume of 770 mL, at which leveling to pH ∼2.6 was noted. This pH was considered the lower limit of the pH meter. In addition, theoretical pKa points were extracted from the program “Chemaxon” (https://chemaxon.com/ ) using the tool chemicalize.com .
6
Determination of Meat Sample pH
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The method by Trout et al. (11 (link)) was followed to determine the pH of the meat samples. The meat samples (10 g) were blended with 50 ml of distilled water for 1 min using a pestle and mortar. The pH was recorded by dipping the glass electrodes of the pH meter (Cyber Scan pH 510, Eutech Instruments; Thermo Fisher Scientific, Navi Mumbai, Maharashtra, India) directly in the suspension.
7
Optimizing EGCG Eye Drops for Animal Study
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The optimal concentration of the various tested concentrations of EGCG was determined to be 20 µg/mL EGCG based on cytotoxicity and anti-inflammatory results. This concentration was added to the buffer as eye drops for the animal study. To assess physical properties, the pH and osmolality of the eye drops containing GE/GEH NPs were determined using a pH meter (CyberScan PH 510; Eutech Instruments, Singapore) and a model 3,320 micro-osmometer (Advanced Instruments, Norwood, MA, USA), respectively. RI values of the colloidal solutions were determined by refractometry (DR-A1; Atago, Tokyo, Japan). PBS was used as the basal solution to dilute NPs to the desired concentration. This colloidal solution was sterilized by filtration through a 0.22 µm filter for the animal study. The eye drops did not contain preservative.
8
Acidifying Activity of Milk Cultures
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Acidifying activity was determined by recording the pH variations of milk cultures. Actively growing cultures were inoculated (2%) in 100 ml of sterile 11% reconstituted skimmed milk (RSM) (w/ v), (RSM, Subotica, Serbia) and the pH measured (pH metre, glass electrode, EUTECH Instruments, CyberScan pH 510, Bukit Raja, Malaysia) at time 0 and after 6 and 24 h of incubation at 30 C. The same procedure was repeated three times for each strain.
9
Offline Shake Flask Cultivation Analysis
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For offline analysis of shake flask experiments, samples were taken from separate Erlenmeyer flasks sealed with cotton plugs. Every flask was only used for one sample, according to Wewetzer et al. [66 (link)]. These conventional shake flasks were cultivated under the same conditions as the RAMOS flasks. Before each flask was filled, main culture medium and the appropriate amount of preculture were mixed in one vessel (master mix) to ensure homogenous conditions in all flasks. The pH-value of the culture broth was measured with a CyberScan pH 510 device (Eutech Instruments, The Netherlands).
The optical density of culture broth was measured at a wavelength of 600 nm in standard 1 cm cuvettes in a photometer (Genesys 20, Thermo Scientific, Germany). To keep OD600 in the linear range of the photometer, samples were diluted with 0.9% NaCl solution if exceeding OD = 0.3.
To determine concentrations of glucose and overflow metabolites (acetate, acetoin, 2,3-butanediol) samples were analyzed by HPLC (Ultimate 3000, Dionex, USA) equipped with an organic acid-resin column (250 × 8 mm, CS-Chromatographie Service GmbH, Langerwehe, Germany) and a Shodex RI-101 refractometer (Showa Denko Europe, Germany). The column was eluted with the mobile phase 5 mM H2SO4 at 60 °C at a flow rate of 0.8 mL/min.
The optical density of culture broth was measured at a wavelength of 600 nm in standard 1 cm cuvettes in a photometer (Genesys 20, Thermo Scientific, Germany). To keep OD600 in the linear range of the photometer, samples were diluted with 0.9% NaCl solution if exceeding OD = 0.3.
To determine concentrations of glucose and overflow metabolites (acetate, acetoin, 2,3-butanediol) samples were analyzed by HPLC (Ultimate 3000, Dionex, USA) equipped with an organic acid-resin column (250 × 8 mm, CS-Chromatographie Service GmbH, Langerwehe, Germany) and a Shodex RI-101 refractometer (Showa Denko Europe, Germany). The column was eluted with the mobile phase 5 mM H2SO4 at 60 °C at a flow rate of 0.8 mL/min.
10
Capillary Electrophoresis for Method Development
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3D-CE capillary electrophoresis system of Agilent Technologies (Santa Clara, CA, USA) was used for method development and validation. This system was equipped with a PDA detector. The capillary zone electrophoresis separation was executed on a fused-silica capillary (total length: 35 cm, effective length: 30 cm, and inner diameter: 50 µm). Software ChemStation version B.03.01 of Agilent Technologies (Santa Clara, CA, USA) was used for data processing.
To measure and adjust the pH of sodium tetraborate solution, a pH meter CyberScan pH 510 of Eutech Instruments was used (Eutech Instruments Pte. Ltd., Singapore).
To measure and adjust the pH of sodium tetraborate solution, a pH meter CyberScan pH 510 of Eutech Instruments was used (Eutech Instruments Pte. Ltd., Singapore).
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