KATOIII, MKN-28 and SGC-7901 GC cell lines were purchased from Shanghai Institute of Biochemistry and Cell Biology (China). Concanavalin A-Sepharose and ion exchange columns were purchased from Pharmacia (Kalamazoo, MI, United States). Markers for sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) assay and bovine serum albumin were purchased from Zhongshan Biological Technology (Beijing, China). Phytohemagglutinin, ammonium persulfate, and a-pyran-1-methyl mannose were purchased from Fluka (part of Sigma-Aldrich, St Louis, MO, United States). Monoclonal antibody against HSP-gp96 was purchased from Santa Cruz Biotechnology (Dallas, TX, United States). Glycine, acrylamide, and ammonium sulfate were purchased from Sigma-Aldrich. Cytokine kits for interleukin (IL)10, IL-12 (p70), interferon (IFN)-γ and tumor necrosis factor (TNF)-α were purchased from R&D Systems (Minneapolis, MN, United States). Flow cytometry reagents and antigens were purchased from BD Pharmingen (San Jose, CA, United States). Lactate dehydrogenase (LDH) release kit was purchased from Promega Biological Technology (Madison, WI, United States).
Kato 3
Manufactured by Shanghai Institute of Biochemistry and Cell Biology
Sourced in China
The KATO-III is a cell line derived from a human gastric carcinoma. It is commonly used in research applications to study gastric cancer and related cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Hgc27, by Shanghai Institute of Biochemistry and Cell Biology (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Nci n87, by Shanghai Institute of Biochemistry and Cell Biology (3 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Bgc 823, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions)
Sgc 7901, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions)
Snu 1, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions)
Snu 16, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions)
Ges cells, by Shanghai Institute of Biochemistry and Cell Biology (2 mentions)
Mkn28, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
Ammonium sulfate, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modi ed eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
4 protocols using kato 3
1
Characterization of Gastric Cancer Cell Lines
2
Cultivation of Gastric Cell Lines
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GC cell lines (KATO-III, SGC-7901, BGC-823, HGC-27, AGS, NCI-N87, SNU-1 and SNU-16) and normal gastric epithelial cells (GES cells) were bought from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). All the cells were cultured at 37°C in a humidified atmosphere containing 5% of CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% of fetal bovine serum (FBS),100 U/ml penicillin, and 100 µg/ml streptomycin (all from Gibco, Invitrogen Life Technologies, Carlsbad, CA, USA).
3
Gastric Cancer Cell Line Transfection Analysis
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The gastric cancer cell lines (HGC-27, NCI-N87, SNU-5 and KATO-III) were purchased from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China), along with human gastric mucosal epithelial cell line GES-1 which was used as a control. Cell lines were grown in RPMI 1640 medium (Invitrogen, USA) containing 10% fetal bovine serum (FBS) (Invitrogen, USA), in a 5% CO2 atmosphere at 37°C. Transfection of the cells was performed using LipofectamineTM 3000 (ThermoFisher Scientific, USA) by following the manufacturer’s instructions. The sequence used in transfection, including the ASMTL-AS1-expressed pcDNA3.1(+) vectors (ov-ASMTL-AS1), empty pcDNA3.1(+) vector (ov-NC), miR-1270 mimic, and mimic negative control, was from Genebio (Shanghai, China). The effect was examined after 48 hours of transfection by quantitative reverse transcription-polymerase chain reaction (RT-qPCR) assay using the extracted RNA.
4
Gastric Cancer Tissue Samples and Cell Lines
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Primary GC tissue samples and adjacent normal tissues were obtained from 40 patients who underwent surgical resection at the Second A liated Hospital of Nanchang University. None of the patients had received preoperative chemotherapy, radiotherapy, or other anticancer modalities. After the resection, the tissue samples were immediately frozen in liquid nitrogen and, then, stored in liquid nitrogen until subsequent treatment and analysis. Eight GC cell lines-KATO-III, SGC-7901, BGC-823, HGC-27, AGS, NCI-N87, SNU-1, and SNU-16-as well as normal gastric cells (GES cells) were bought from the Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). All the cells were cultured at 37°C in a humidi ed atmosphere containing 5% of CO2 in Dulbecco's Modi ed Eagle's Medium (DMEM) supplemented with 10% of fetal bovine serum (FBS),100 U/ml penicillin, and 100 µg/ml streptomycin (all from Gibco, Invitrogen Life Technologies, Carlsbad, CA, USA).
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