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2 protocols using human catalog i1395 il 6

1

Investigating Immune Modulation in Preclinical Models

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Mouse (Catalog I9646) and human (Catalog I1395) IL-6 were from MilliporeSigma. Mouse (Catalog 713704) and human (Catalog 713604) GM-CSF and mouse (Catalog 713502) and human (Catalog 713402) G-CSF were purchased from Biolegend. The T cell proliferation dye used was CellTrace Calcein Violet (ThermoFisher Scientific, Catalog C34858). propranolol (Catalog P0884) and isoproterenol (Catalog I6504) were purchased from MilliporeSigma. In vitro, propranolol was used at a concentration of 1 μM and isoproterenol was used at a concentration of 10 μM. Etomoxir (Tocris, Catalog 4539) was used in vivo at a dose of 10mg/kg and in vitro at a concentration of 10 μM. To stain MDSCs for autophagic vesicles, the CYTO-ID® Autophagy detection kit 2.0 (Catalog ENZ-51031-0050) from Enzo Life Sciences was used. LPS (MilliporeSigma, Catalog L3024) was used at a concentration of 10ng/ml. Torin-1 (Cell Signaling, Catalog 14379S) was used at a concentration of 250 nM. 3-MA (Torics, Catalog 3977) was used at a concentration of 2 mM. Chloroquine (Enzo Life Sciences, Catalog 51031) was used at a concentration of 10 μM.
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2

Investigating Immune Modulation in Preclinical Models

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse (Catalog I9646) and human (Catalog I1395) IL-6 were from MilliporeSigma. Mouse (Catalog 713704) and human (Catalog 713604) GM-CSF and mouse (Catalog 713502) and human (Catalog 713402) G-CSF were purchased from Biolegend. The T cell proliferation dye used was CellTrace Calcein Violet (ThermoFisher Scientific, Catalog C34858). propranolol (Catalog P0884) and isoproterenol (Catalog I6504) were purchased from MilliporeSigma. In vitro, propranolol was used at a concentration of 1 μM and isoproterenol was used at a concentration of 10 μM. Etomoxir (Tocris, Catalog 4539) was used in vivo at a dose of 10mg/kg and in vitro at a concentration of 10 μM. To stain MDSCs for autophagic vesicles, the CYTO-ID® Autophagy detection kit 2.0 (Catalog ENZ-51031-0050) from Enzo Life Sciences was used. LPS (MilliporeSigma, Catalog L3024) was used at a concentration of 10ng/ml. Torin-1 (Cell Signaling, Catalog 14379S) was used at a concentration of 250 nM. 3-MA (Torics, Catalog 3977) was used at a concentration of 2 mM. Chloroquine (Enzo Life Sciences, Catalog 51031) was used at a concentration of 10 μM.
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