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Vh z25 lens

Manufactured by Keyence
Sourced in Japan

The VH-Z25 lens is a high-performance optical lens designed for use with Keyence's VH series of microscopes. The lens provides a magnification range of 2.5x to 25x, enabling users to obtain detailed and clear images of their specimens. The VH-Z25 lens is built with high-quality optics to deliver consistent and reliable performance.

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3 protocols using vh z25 lens

1

Depth Composition Imaging of Tooth Surface

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Images were taken of the sample region of interest using a digital microscopy/3D surface profilometry system, the VHX-1000 from Keyence (Elmwood, NJ) outfitted with the VH-Z25 lens (magnification from 25–175×). Depth composition images were generated by translating the image plane of the objective along the vertical axis capturing a series of images containing all points of the tooth surface in optimum focus, followed by construction of an optimized 2D image using only in focus image regions from the series.
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2

Isolation and Characterization of Fungal Metabolites

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To confirm the AF productivity of the FUT-A1 strain and other fungi isolated from soils of Fukui city, Japan, we cut out an aliquot of the fungal colony together with the agar underneath the colony on an agar plate and transferred it to a new 1.5 mL microtube. Metabolites in the samples were extracted with 0.3 mL of ethyl acetate followed by mixing with a vortex mixer. After centrifugation at 10,000× g for 2 min, the upper extract (10 μL) was analyzed by TLC using a silica gel plate (silica gel 60, #5721; Merck, Rahway, NJ, USA) and a developing solution of toluene-ethyl acetate-acetic acid (60:30:4, v/v/v).
For the determination of the morphological characters, each fungus was inoculated on PDA medium and cultured for 4 days. The colony was observed using a VHX digital microscope equipped with a VH-Z25 lens (Keyence, Osaka, Japan).
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3

Digital Microscopy for Quantifying Enamel Lesions

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Images were taken of the region of interest on each tooth surface using a digital microscopy/3D surface profilometry system, the VHX-1000 from Keyence (Elmwood, NJ) outfitted with the VH-Z25 lens (magnification from 25–175×). Depth composition images (DCDM) were generated by translating the image plane of the objective along the vertical axis capturing a series of images containing all points of the tooth surface in optimum focus, followed by construction of an optimized 2D image using only in focus image regions from the series.
The lesion contrast measurements from visible light images where calculated by averaging the pixel values taken from a ROI approximately 25 × 25-pixels from areas of sound enamel, demineralized enamel and the composite regions. The contrast of the decay relative to both the adjacent sound enamel and restorations were calculated using the formula (IL-IS/C)/IL, where IL represents the lesion intensity and IS/C represents either the intensity from the sound or composite regions. The image contrast varies from 0 to 1 with 1 being very high contrast and 0 having no contrast.
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