4-wk-old female ddY and BALB/c mice (specific pathogen-free) were purchased from Japan SLC Co. Ltd. Testing the pathogenicity of the viruses used in the present study revealed that BALB/c mice tended to be more susceptible compared with ddY, and thus, they were used in the full-scale evaluation of the VLP vaccine.
Balb c mice
Manufactured by Japan SLC
Sourced in Japan, United States
BALB/c mice are an inbred strain of laboratory mice commonly used in research. They are characterized by a white coat color and are known for their susceptibility to certain types of cancer and immune system disorders. BALB/c mice are widely used in various fields of biomedical research, including immunology, oncology, and infectious disease studies.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6 mice, by Japan SLC (6 mentions)
C57bl 6j, by Japan SLC (4 mentions)
Balb c nu nu mice, by Japan SLC (3 mentions)
Rpmi 1640 medium, by Fujifilm (3 mentions)
Fetal bovine serum (fbs), by Corning (3 mentions)
C57bl 6j mice, by Japan SLC (2 mentions)
Sjl j mice, by Charles River Laboratories (2 mentions)
Male sprague dawley rats, by Japan SLC (2 mentions)
Wistar rats, by Japan SLC (2 mentions)
Sd rats, by Japan SLC (2 mentions)
C57bl 6, by Japan SLC (2 mentions)
Streptomycin, by ICN Biomedicals (2 mentions)
Penicillin, by ICN Biomedicals (2 mentions)
Hank s balanced salt solution, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Renca, by American Type Culture Collection (1 mentions)
α mem, by Nacalai Tesque (1 mentions)
Isocage, by Tecniplast (1 mentions)
Macs na ve cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions)
40 μm cell strainer, by BD (1 mentions)
138 protocols using balb c mice
1
Pathogenicity Evaluation of VLP Vaccine
2
Characterization of Histamine Receptor Knockout Mice
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Specific-pathogen-free 5–10-week-old male BALB/c mice and C57BL/6 mice were obtained from Japan SLC (Hamamatsu, Japan). Hdc−/− mice [32 (link)] were backcrossed to BALB/c strain for more than 10 generations. Hh1r−/− mice [16 (link)] were backcrossed to C57BL/6 strain for more than 10 generations. These gene-targeted male mice were used at 8–10 weeks of age. All mice were kept in a specific-pathogen-free animal facility at Okayama University. This study was approved by the Committee on Animal Experiments of Okayama University (OKU-201269; June 2012–March 2015, OKU-2015037; April 2015–March 2018, and OKU-2018178; April 2018–March 2021).
3
Murine Tumor Models for Immunotherapy
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Murine colon carcinoma CT26 (CRL-2638) and Colon26, breast cancer 4T1 (CRL-2539) and EMT6 (CRL-2755), and renal adenocarcinoma Renca (CRL-2947) cells were obtained from American Type Culture Collection (Manassas, Virginia, USA). BALB/c mice (6 weeks old, female) were purchased from Japan SLC (Shizuoka, Japan). CT26, 4T1, and Renca cells were cultured in RPMI 1640 (Sigma-Aldrich, St. Louis, Missouri, USA) supplemented with 10% fetal bovine serum (FBS; Biowest, Nuaille, France) and 1% penicillin and streptomycin (P/S; Nacalai Tesque, Kyoto, Japan). EMT6 cells were cultured in α-MEM (Nacalai Tesque) supplemented with 10% FBS and 1% P/S. Mycoplasma was not detected in the culture, and the cells were used for experiments within 20 passages after procurement. Cancer cells were subcutaneously transplanted into syngeneic mice using 1.5×106 cells in 100 µL Hanks’ balanced salt solution (Gibco, Carlsbad, California, USA).
4
BALB/c Mice Infection Protocol
5
Genetic Modeling of IL-6 Deficiency
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Pathogen-free male BALB/c mice that are 8–10 weeks old were obtained from Japan SLC (Shizuoka, Japan) and were designated as WT mice in this study. Il6−/− mice were a kind gift of Dr. Blüthmann (24 (link)) and were back-crossed to BALB/c mice for more than 10 generations. Subsequently, age- and sex-matched Il6−/− mice were used in these experiments (25 (link)). All mice were housed individually in cages under specific pathogen-free conditions during the experiments. All animal experiments were approved by the Committee on Animal Care and Use in Wakayama Medical University and complied with the Guidelines for the Care and Use of Laboratory Animals of Wakayama Medical University.
6
Differentiation of Th2 Cells from Naïve CD4+ T Cells
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Five-week-old male BALB/c mice (weighing 18–20 g) were obtained from Japan SLC, Inc. (Shizuoka, Japan) and maintained in a controlled, specific pathogen-free environment (temperature, 24 °C ± 2 °C; humidity, 50% ± 5%; and light/dark cycle, 12/12 h with food and water provided ad libitum). Spleen tissues were passed through 40-μm cell strainers (Falcon, New York, NY, USA), and red blood cells were removed using a specific lysis buffer (BioLegend, San Diego, CA, USA). Naïve CD4+ T cells were isolated from the splenocytes using the Naïve CD4+ T Cell Isolation Kit (MACS; Miltenyi Biotec Inc., Auburn, CA, USA) and were differentiated into Th2 cells using the CellXVivo Mouse Th2 Cell Differentiation Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturers’ instructions. The animal experiments for the differentiation of Th2 cells from naïve CD4+ T cells were approved by the institutional animal care committee of Korea Institute of Oriental Medicine (KIOM: #20-070).
7
Establishment of Metastatic Mammary Carcinoma Cell Line
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Mammary tumors arising from BJMC3879 cell implantation have mutant p53 and an especially high metastatic predilection for the lymph nodes and lungs,18 , 19 , 20 a trait retained through culture. To monitor the in vivo progression and dissemination, we performed a stable transfection of the BJMC3879 parent cell line with luc2, an improved firefly luciferase gene, to generate the BJMC3879Luc2 mammary carcinoma cell line.21 BJMC3879Luc2 cells used in this study were maintained in DMEM with 10% FBS and penicillin‐streptomycin and grown in an incubator under 5% CO2 at 37°C.
Forty 5‐week‐old female BALB/c mice were purchased from Japan SLC, Hamamatsu, Japan. The animals were housed 5 to plastic cage on wood chip bedding with free access to water and food under controlled temperature (21 ± 2°C), humidity (50% ± 10%) and lighting (12‐12 hours light‐dark cycle). All animal experiments were approved by the Institutional Review Board of the Osaka Medical College (approval no. 20023) and were performed in accordance with the procedures outlined in the Guide for the Animal Care and Use of Laboratory Animals of Osaka Medical College.
Forty 5‐week‐old female BALB/c mice were purchased from Japan SLC, Hamamatsu, Japan. The animals were housed 5 to plastic cage on wood chip bedding with free access to water and food under controlled temperature (21 ± 2°C), humidity (50% ± 10%) and lighting (12‐12 hours light‐dark cycle). All animal experiments were approved by the Institutional Review Board of the Osaka Medical College (approval no. 20023) and were performed in accordance with the procedures outlined in the Guide for the Animal Care and Use of Laboratory Animals of Osaka Medical College.
8
BALB/c Mice Acclimatization Protocol
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Five-week-old female BALB/c mice were purchased from Japan SLC (Shizuoka, Japan) and acclimatized for 1 week. The animals were housed under specific
pathogen-free conditions. All experiments were approved by the Institutional Animal Care and Use Committee for Kitasato University Medical Center (No. 24-4),
and animals were treated in accordance with the Regulations for Animal Experiments in Kitasato University.
pathogen-free conditions. All experiments were approved by the Institutional Animal Care and Use Committee for Kitasato University Medical Center (No. 24-4),
and animals were treated in accordance with the Regulations for Animal Experiments in Kitasato University.
9
Comparison of BALB/c and C57BL/6J Mice
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BALB/c mice (females, 6 week-old) and C57BL/6J mice (females, 6 week-old) were purchased from Japan SLC, Inc. (Shizuoka, Japan). Protocols for the animal experiments were reviewed and approved by the Chiba University Animal Care Committee in accordance with the “Guide for Care and Use of Laboratory Animals”.
10
BALB/c Mice for Pathogen-free Experiments
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Female young BALB/c mice and retired breeder BALB/c mice were obtained from Japan SLC
(Hamamatsu, Japan) and were used for experiments when aged 10 or 40 weeks, respectively.
These mice had been bred under specific pathogen-free conditions in the animal facility of
Kitasato University Medical Center. All the experiments were approved by the Institutional
Animal Care and Use Committee for Kitasato University Medical Center (approval number:
H19-002A), and the animals were treated in accordance with the Regulations for Animal
Experiments in Kitasato University.
(Hamamatsu, Japan) and were used for experiments when aged 10 or 40 weeks, respectively.
These mice had been bred under specific pathogen-free conditions in the animal facility of
Kitasato University Medical Center. All the experiments were approved by the Institutional
Animal Care and Use Committee for Kitasato University Medical Center (approval number:
H19-002A), and the animals were treated in accordance with the Regulations for Animal
Experiments in Kitasato University.
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