The soil moisture content of the control and drought-stressed plots was measured using a soil moisture meter (TDR 300, Spectrum Technologies, Plainfield, IL, USA).
Tdr 300
Manufactured by Spectrum Technologies
Sourced in United States
The TDR 300 is a time-domain reflectometer, a device used to locate and identify faults in electrical cables and transmission lines. It works by sending a pulse down the cable and measuring the time it takes for the pulse to reflect back, which allows it to determine the location of any discontinuities or defects in the cable.
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Lab products found in correlation
7 protocols using tdr 300
1
Measuring Soil Moisture in Drought Trials
2
Soil Water Content and Temperature Monitoring
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Volumetric soil water content (SWC) was measured at 10 cm by a time domain reflectrometry (TDR 300, Spectrum Technologies, Inc. CST, USA) that was inserted in the soil vertically. Soil temperature (ST) at a depth of 10 cm below the surface was monitored by using a thermometer set in the center of each plot (Model TL-883, Tonglixing technology Co., Ltd., China).
3
Drought and Methyl Jasmonate Response in Trifoliate Orange
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Six-month-old trifoliate orange (Poncirus trifoliate) seedlings in this assay were planted in a green house. All tobacco (Nicotiana benthamiana) seedlings were grown in a plant growth chamber with 16 h light/8 h dark cycle at 28 °C.
For drought treatment, trifoliate orange seedlings planted in a premix substrate were treated with no watering. Samples were collected when the relative water content of the substrate descended to 70%, 60%, 50%, 40%, 30% and 20%, respectively, detected by a soil moisture meter TDR 300 (Spectrum Technologies, Alpharetta, GA, USA). Control samples (CK) were collected from well-watered seedlings.
One mM MeJA was sprayed on the leaves of trifoliate orange. Samples were collected at 0, 12, 24 and 72 h after treatment. All samples were stored at −80 °C until use.
For drought treatment, trifoliate orange seedlings planted in a premix substrate were treated with no watering. Samples were collected when the relative water content of the substrate descended to 70%, 60%, 50%, 40%, 30% and 20%, respectively, detected by a soil moisture meter TDR 300 (Spectrum Technologies, Alpharetta, GA, USA). Control samples (CK) were collected from well-watered seedlings.
One mM MeJA was sprayed on the leaves of trifoliate orange. Samples were collected at 0, 12, 24 and 72 h after treatment. All samples were stored at −80 °C until use.
4
Soil Moisture Monitoring Protocol
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We used precipitation and air temperature data from a meteorology station ~18 km away from the experimental site. Soil moisture was measured every seven days from June to September at 3.8, 7.6, and 12 cm depths in all 20 plots using the TDR 300 (Spectrum Technologies, Plainfield, Illinois, U.S.A.).
5
Soil Respiration Measurement Technique
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Rs was measured in situ every 10 days from September 2017 to October 2018 using LI-8100 (Li-COR Inc., Lincoln, Nebraska, USA). Two polyvinyl chloride collars of 7 cm height (inserted ~4 cm into the soil) and 20 cm inner diameter for Rs measurements were permanently installed in each chamber 3 months before the first measurement to minimize disturbance. Rs was measured between 9:00 and 11:00 am (local time) on rainless mornings. For each collar, Rs was determined twice, and the average was used as the measured value for the collar. Each Rs measurement in elevated CO 2 chambers was carried out at least 2 h after the CO 2 enrichment was switched off because a higher headspace concentration in elevated CO 2 chambers could suppress the diffusion of CO 2 from soils [33] (link). During each Rs measurement, 10 cm underground soil temperature and moisture were measured at three random places in each chamber. Soil temperature was determined by a Li-COR thermocouple probe, and soil moisture was measured using a TDR 300 (Spectrum Technologies Inc., South Bend, IN, USA). Rs in each chamber was represented by the average of measurements from two collars. Both soil temperature and moisture in each chamber were expressed as the average of measurements from three places.
6
Assessing Urban Tree Ecosystem Services
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On each pair of paved and vegetated plots, we measured DBH, height, crown radius, and leaf area density (LAD; total one-sided leaf area per unit volume) of 560 individuals of G. biloba and P. orientalis in both years of 2018 and 2021 at the interval of 3 years. DBH was measured with a steel dendrometer band at 1.3 m aboveground, except in the case of buttressed stems, which were measured above the buttress to avoid overestimation (Condit, 1998 ). Height was determined as the height from the root collar to the top of the highest living bud using a Nikon Forestry Pro (Nikon Vision, Tokyo, Japan) laser rangefinder to the accuracy of 0.1 m. Crown radius was the average distance from the center of the trunk to the farthest point below branch tips in four directions, north, south, east, and west, which was measured using a linear tape. LAD was measured by the LAI-2000 Plant Canopy Analyzer (Li-Cor, Inc., Lincoln, NE, USA).
At each pit where trees were measured, the surface temperature, soil temperature, and soil moisture were remeasured during each investigation. The surface temperature and soil temperature were measured using an infrared thermometer (Optris MS, Optris GmbH, Berlin, Germany) and soil thermometer (SYS-TP101, SYS, Liaoning, China). The soil moisture was measured using TDR300 (Spectrum Technologies Inc., Plainfield, IL, USA).
At each pit where trees were measured, the surface temperature, soil temperature, and soil moisture were remeasured during each investigation. The surface temperature and soil temperature were measured using an infrared thermometer (Optris MS, Optris GmbH, Berlin, Germany) and soil thermometer (SYS-TP101, SYS, Liaoning, China). The soil moisture was measured using TDR300 (Spectrum Technologies Inc., Plainfield, IL, USA).
7
Soil Moisture, Temperature, and Chemistry Analysis
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A TDR-300 (produced by Spectrum Technologies in Plainfield, IL, USA) is utilized to monitor soil moisture levels within a 0–10 cm depth. Meanwhile, the LI-8100 instrument (manufactured by LI-COR in Lincoln, NE, USA) measured the soil temperature within the same depth range. For pH measurements, a pH meter (model FE20-FiveEasy pH, from Mettler Toledo in Gießen, Germany) was employed after mixing the soil with water at a ratio of 1:2.5. To determine total carbon (TC) and total nitrogen (TN) content, an Elemental Analysis System (Vario EL III, Elemental Analysis System GmbH, Langenselbold, Germany) was used [34 (link)].
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