Ringer's solution, containing 122 mM sodium chloride, 3 mM potassium chloride, 0.4 mM monopotassium phosphate, 1.2 mM magnesium sulfate, 25 mM sodium bicarbonate, and 1.2 mM calcium chloride, was purchased from Millipore (MA, USA). Pachymic acid, liquiritin, rhynchophylline, isorhynchophylline, corynoxeine, and isocorynoxeine (purity ≥ 99%) were purchased from National Institutes for Food and Drug Control (Beijing, China). Fetal bovine serum (FBS) was purchased from Gibco/BRL (Grand Island, NY, USA). Methyl thiazolyl tetrazolium (MTT), dimethyl sulfoxide (DMSO), and trypsin were provided by Sigma Chemical (St. Louis, MO, USA). Aβ1–42 (purity ≥ 95%) was purchased from Abcam (Cambridge, USA). HPLC-grade acetonitrile was purchased from Thermo Fisher Scientific (Massachusetts, USA). EliVision plus and DAB kits were provided by Fuzhou Maixin Biotech. Co., Ltd. (Fuzhou, China). Bax, Bcl-2, caspase-3 and caspase-9 antibodies, annexin V-FITC/PI assay kit, basal DMEM medium, cell lysis solution, and 0.1% DEPC water were purchased from Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China). Nimodipine was provided by Bayer healthcare Co., Ltd. (Leverkusen, Germany).
Cell lysis solution
Manufactured by Keygen Biotech
Sourced in China
The Cell Lysis Solution is a reagent designed to disrupt the cell membrane and release the cellular contents, including proteins, nucleic acids, and other biomolecules. It is a key component in various biological and biochemical applications that require the extraction and isolation of cellular components.
Automatically generated - may contain errors
Lab products found in correlation
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Aβ1 42, by Abcam (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Merck Group (1 mentions)
Bcl 2, by Keygen Biotech (1 mentions)
Bcl2 associated x (bax), by Keygen Biotech (1 mentions)
Annexin 5 fitc pi assay kit, by Keygen Biotech (1 mentions)
Amersham imager 600 instrument, by GE Healthcare (1 mentions)
2 protocols using cell lysis solution
1
Neuroprotective Potential of Natural Compounds
2
Protein Expression Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell lysis solution (Nanjing KeyGen Biotech Co., Ltd, Nanjing, China) was added, the dish agitated for 30 min, centrifuged at 12,000 × g at 4°C for 15 min and the supernatant removed. Detection of protein concentration was conducted using BCA protein quantitative kits. Protein extracts (20 µg) were separated by 8–10% SDS polyacrylamide gel electrophoresis, then transferred to nitrocellulose membranes and blocked by 10% skim milk for 1.5 h at 4°C, then incubated by the corresponding antibody at 4°C overnight (HIF-1α, 1:1,000; Twist, 1:500; E-cad, 1:200; N-cad, 1:1,000; and β-actin, 1:1,500), β-actin was used as an internal control. The second antibody incubation was at room temperature for 1 h, followed by ECL chemiluminescence reagent chromogenic (cat. no. MA02454; Thermo Fisher Scientific, Inc.) for 1 min and exposure in an Amersham Imager 600 instrument (GE Healthcare Life Sciences, Little Chalfont, UK). This was followed with grey value analysis with Quantity one, the relative grayscale value indicating the corresponding protein expression level.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!