Mda mb 453 htb 131

The MDA-MB-453 (HTB-131, RRID:CVCL_0418), MDA-MB-231 (HTB-26, RRID:CVCL_0062), MDA-MB-468 (HTB-132, RRID:CVCL_0419) and HEK 293 T/17 (CRL-11268G-1, RRID:CVCL_UE07) cell lines were obtained from the American Type Culture Collection (ATCC, USA) and the MFM-223 line (ACC-420, RRID:CVCL_1408) from the DSMZ-German Collection of Microorganisms and Cell Cultures (Germany, RRID:SCR_001711). All cell culture reagents were purchased from Sigma-Aldrich (USA). MDA-MB-453, MDA-MB-231, MDA-MB-468, and 293 T/17 cells were maintained in DMEM High Glucose medium supplemented with 10% (v/v) FBS and 2 mM L-Glutamine. MFM-223 cells were maintained in EMEM supplemented with 10% FBS, 2 mM L-Glutamine and 1x Insulin–Transferrin–Sodium Selenite Supplement. Cell lines were cultured at 37 °C in a humidified incubator containing 5% CO₂, confirmed negative for mycoplasma contamination via testing with an IP-protected Mycoplasma spp. detection assay developed in house and authenticated by short tandem repeat profiling (CellBank Australia (RRID:SCR_013086)).

Human breast adenocarcinoma cells [MDA-MB-231 (HTB-26™) and MDA-MB-453 (HTB-131™)] and mouse macrophages (RAW 264.7) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). MDA-MB-231 and MDA-MB-453 cells were maintained in Leibovitz’s L-15 medium (WELGENE, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS, WELGENE), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco, Waltham, MA, USA) in a humidified and carbon dioxide (CO2)-free atmosphere at 37 °C. RAW 264.7 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; WELGENE) supplemented with 10% FBS (WELGENE), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Gibco) in a humidified atmosphere of 95% air and 5% CO₂ at 37 °C.