Sw684

The normal fibroblast cells human dermal fibroblast primary cell (hDFPC), human lung fibroblasts primary cell (hLFPC), human mammary fibroblasts primary cell (hMFPC), human embryonic lung fibroblast (HFL1), and one fibrosarcoma cell HT-1080 were obtained from Procell (Wuhan, China). Another fibrosarcoma cell SW684 was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). All cells cultured at 37°C in a humidified atmosphere with 5% CO₂, and in different complete medium. hDFPC(CM-H103), hLFPC(CM-H011), hMFPC(CM-H172), HFL1(CM-H159), HT-1080(CM-0117), and SW684 was routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM) medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and penicillin/streptomycin (i.e., complete medium).

HT1080, SKUT1, sNF96.2, 93T449, SW684, SW872 and SW982 were purchased from the American Type Culture Collection (ATCC, Teddington, Middlesex, UK), and cultured according to their recommendations. Cell lines and culture conditions are summarized in Supplementary Table 1. Cell lines were authenticated by the Promega Powerplex 21 System and underwent mycoplasma screening (Molecular Biology Core Facility, CRUK Manchester Institute, UK).