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Nci h1568

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The NCI-H1568 is a cell line derived from a human lung adenocarcinoma. It is available for purchase from the American Type Culture Collection (ATCC) for research purposes.

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Lab products found in correlation

Nci h23, by American Type Culture Collection (4 mentions) Nci h460, by American Type Culture Collection (3 mentions) Nci h1437, by American Type Culture Collection (3 mentions) Nci h1975, by American Type Culture Collection (2 mentions) Pen strep, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Nci h1299, by American Type Culture Collection (2 mentions) Nci h522, by American Type Culture Collection (1 mentions) Nci h1792, by American Type Culture Collection (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Nci h1048, by American Type Culture Collection (1 mentions) Mia paca 2, by American Type Culture Collection (1 mentions) Nci h1915, by American Type Culture Collection (1 mentions) Hcc1428, by American Type Culture Collection (1 mentions) Begm media, by Lonza (1 mentions) Fetal bovine serum (fbs), by Corning (1 mentions) Nci h292, by American Type Culture Collection (1 mentions) Nci h838, by American Type Culture Collection (1 mentions) Nci h720, by American Type Culture Collection (1 mentions)

6 protocols using nci h1568

1

Characterization of Isogenic KRAS-Mutant Colon and Lung Cancer Cell Lines

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The paired isogenic HCT116 KRASWT/− and HCT116 KRASWT/G13D colon cancer cell lines were kind gifts from Dr. Bert Vogelstein (Johns Hopkins, Baltimore, MD). A549, NCI-358, NCI-H460, NCI-H522, NCI-H1568, NCI-H1792, NCI-H23, NCI-H1975, and NCI-H1437 were obtained from the ATCC (Manassas, VA). HCT116 cells were cultured in DMEM (Invitrogen) with 10% FBS (Invitrogen) and 1% Pen/Strep (Invitrogen). All lung cancer cell lines were cultured in RPMI 1640 (Gibco) with 10% FBS and 1% Pen/Strep. HCT116 Cell lines were validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Strict bio-banking procedures were followed and cells were tested for contamination, including mycoplasma. All other cell lines were obtained from ATCC and used within 2–4 months.
Zhou Y., Dang J., Chang K.Y., Yau E., Aza-Blanc P., Moscat J, & Rana T.M. (2016). miR-1298 inhibits mutant KRAS-driven tumor growth by repressing FAK and LAMB3. Cancer research, 76(19), 5777-5787.
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2

Comprehensive Cell Line Characterization

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The following cell lines were purchased from ATCC: human lung cancer cell lines A549, NCI-H1915, NCI-H1568, NCI-H23, NCI-H1048; human breast cancer cell lines HCC38, HCC1428; human pancreatic cancer cell line: MIAPACA-2. The following cell lines were obtained from the Cancer Cell Line Encyclopedia project (30 (link)): human lung cancer cell lines NCI-H2030, NCI-H3122, NCI-H1792, HARA, human acute myeloid leukemia cell line MOLM13. Human breast cancer cell lines T47D and MCF7, and esophageal cancer cell line OE21 were kindly provided by Dr. Adam Bass (Dana-Farber Cancer Institute). All cell lines were grown at 37°C and 5% CO2 and were confirmed to be Mycoplasma negative. Cell lines were cultured in RPMI supplemented with 10% FBS and 1% penicillin/streptomycin, except for MCF-7 and MIAPACA-2 cell lines that were maintained in DMEM containing 10% FBS and 1% penicillin/streptomycin.
Zhang Z., Golomb L, & Meyerson M. (2022). Functional genomic analysis of CDK4 and CDK6 gene dependency across human cancer cell lines. Cancer research, 82(11), 2171-2184.
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3

Culturing Human Lung Cancer Cell Lines

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Human bronchial epithelial cell line HBE (RRID:CVCL_0287) was a kind gift of Dr. Daniel Weiss (University of Vermont), and was maintained in BEGM media (Lonza,; Allendale, NJ). Human NSCLC lines NCI-H838 (RRID:CVCL_1594) and NCI-H1568 (RRID:CVCL_1476) were purchased from the NCI Division of Cancer Diagnosis and Treatment Tumor Repository (Operated by Charles River Laboratories, Inc.; Wilmington, MA), cell lines A549 (ATCC Cat# CCL-185, RRID:CVCL_0023), NCI-H292 (ATCC Cat# CRL-1848, RRID:CVCL_0455), NCI-H460 (ATCC Cat# HTB-177, RRID:CVCL_0459), and NCI-H1437 (ATCC Cat# CRL-5872, RRID:CVCL_1472) were obtained through the American Type Culture Collection (Manassas, VA), and lines NCI-H720 (RRID:CVCL_1583) and NCI-H1299 (RRID:CVCL_0060) were a kind gift of Dr. Jitesh Pratap (Rush University). All cancer cell lines were cultured in RPMI-1640 media supplemented with 10% fetal bovine serum (Cellgro-Corning Cat. No. 35-010-CV) and 1% penicillin/streptomycin (Hyclone-GE Cat. No. SV30079.01) and incubated in a 37 °C 5% CO2/95% air humidified incubator.
Ramsey J., Butnor K., Peng Z., Leclair T., van der Velden J., Stein G., Lian J, & Kinsey C.M. (2017). LOSS OF RUNX1 IS ASSOCIATED WITH AGGRESSIVE LUNG ADENOCARCINOMAS. Journal of cellular physiology, 233(4), 3487-3497.
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4

Comparative Analysis of NSCLC Cell Lines

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The human NSCLC cell lines, NCI-H1568 and A549 were obtained from the American Type Culture Collection. HCC95, NCI-H3122, NCI-H1650, HCC827, NCI-H1975, normal airway epithelial cell line (SALE) and KRASG12D transformed SALE cells (SALER) were kindly provided by Dr. Thomas Roman. Cancer cells were cultured in RPMI-1640 medium supplemented with 10% FBS, L-glutamine, 100 U/ml penicillin and 50 μg/ml streptomycin. Cell lines were identity-verified by STR analysis and certified as mycoplasma-free within 6 months of the experiments reported. All cell lines were authenticated using the Stem-Elite ID System (Promega, Madison, WI, USA).
Tyrosine kinase inhibitors Gefitinib, Lapatinib, and Erlotinib were purchased from Vichem Chemie. Chemotherapeutic agents Paclitaxel, and putative stem cell killers Metformin and Salinomycin were purchased from Sigma. Afatinib was purchased from SelleckChem, Carboplatin was purchased from Santa Cruz.
Xiao Z., Sperl B., Gärtner S., Nedelko T., Stacher-Priehse E., Ullrich A, & Knyazev P.G. (2019). Lung cancer stem cells and their aggressive progeny, controlled by EGFR/MIG6 inverse expression, dictate a novel NSCLC treatment approach. Oncotarget, 10(26), 2546-2560.
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5

Culturing Lung Adenocarcinoma Cell Lines

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Lung adenocarcinoma cell lines A549, NCI-H23, NCI-H1568, and NCI-H1651 were obtained from American Type Culture Collection (ATCC, Manassas, VA). A549 and H23 cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), penicillin, and streptomycin. H1651 were cultured in DMEM:F12 medium, as recommended by ATCC, with the following modifications. DMEM:F12 culture media was supplemented with EGF (10 ng/mL final concentration), 1% FBS, and penicillin/streptomycin. H1568 cells were cultured using the same DMEM:F12 supplemented media as described for H1651.
Morris B.B., Wages N.A., Grant P.A., Stukenberg P.T., Gentzler R.D., Hall R.D., Akerley W.L., Varghese T.K., Arnold S.M., Williams T.M., Coppola V., Jones D.R., Auble D.T, & Mayo M.W. (2021). MYBL2-Driven Transcriptional Programs Link Replication Stress and Error-prone DNA Repair With Genomic Instability in Lung Adenocarcinoma. Frontiers in Oncology, 10, 585551.
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6

NSCLC Cell Line Panel for Research

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The human NSCLC cell lines HCC827, NCI‐H1437, NCI‐H1734, NCI‐H358, NCI‐H1781, NCI‐H2170, NIC‐H1650, NCI‐H2106, NCI‐H2087, NCI‐H2347, NCI‐H441, Hs 618.T, NCI‐H1299, NCI‐H460, NCI‐H1975, NCI‐H1568, NCI‐H23, Calu‐3, and A549 were obtained from the American Type Culture Collection (Manassas, VA, USA). The human NSCLC cell line COR‐L105 was purchased from Sigma‐Aldrich, and human NSCLC cell line HCC‐15 was purchased from Creative Dynamics (Shirley, NY , USA). The HS618.T cell line was cultured in Dulbecco's Modified Eagle's medium, supplemented with 10% FBS. A549 was cultured in F‐12K medium, supplemented with 10% FBS. NCI‐H2106 was cultured in HITES medium supplemented with 5% FBS. Calu‐3 was cultured in Eagle's Minimum Essential Medium supplemented with 10% FBS. NCI‐H2087 was cultured in RPMI‐1640 medium supplemented with 5% FBS. All other cell lines were maintained in RPMI‐1640 medium supplemented with 10% FBS. Cells were grown at 37 °C in a humidified 5% CO2 : 95% air atmosphere.
Tavassoly I., Hu Y., Zhao S., Mariottini C., Boran A., Chen Y., Li L., Tolentino R.E., Jayaraman G., Goldfarb J., Gallo J, & Iyengar R. (2019). Genomic signatures defining responsiveness to allopurinol and combination therapy for lung cancer identified by systems therapeutics analyses. Molecular Oncology, 13(8), 1725-1743.
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