Natriumazide

Deionized water was obtained from a Milli-Q^® Integral purification system from Merck KGaA (Darmstadt, Germany). Natriumazide and sodium sulphate were from Carlo Erba Reagenti (Milan, Italy). Potassium dihydrogen phosphate, ribonuclease A, β-lactoglobulin, bovine serum albumin, sodium alginate, sodium carbonate, lithium bromide, calcium chloride, protamine, potassium chloride, and thyroglobulin were from Sigma–Aldrich (Milan, Italy). Rituximab was purchased as MabThera from Roche (Basel, Switzerland).

Acetone, Azide-fluor 545, collagenase IA, curcumin, dimethylsulfoxide (DMSO), glutaraldehyde, ethanol, lithium bromide, methanol, potassium chloride, potassium dihydrogen phosphate, sodium carbonate, sodium hydroxide, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and Millipore’s Amicon^® Ultra filters were purchased from Sigma-Aldrich (Milan, Italy). Dialysis cellulose tubes were purchased from Visking, Medicell Membranes Ltd. (London, UK). Moreover, 70 μm nylon meshes were purchased from Greiner Bio-One GmbH (Kremsmunster, Austria). Natriumazide and sodium sulphate were from Carlo Erba Reagenti (Milan, Italy). Caucasian colorectal adenocarcinoma cells (Caco-2), human cervix cancer cells (HeLa) and human urinary bladder (ECV) cancer cells were purchased from the European Collection of Authenticated Cell Cultures Cell Bank (ECACC, Salisbury, UK). All reagents used for cell cultures were purchased from Euroclone (Milan, Italy).