Log In Sign up
The largest database of trusted experimental protocols

Mouse anti smad2 3

Manufactured by BD
Visit Supplier
Sourced in United States

Mouse anti-Smad2/3 is a primary antibody that recognizes both Smad2 and Smad3 proteins. Smad2 and Smad3 are intracellular proteins that are critical mediators of transforming growth factor-beta (TGF-β) signaling.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant tgf β tgf β3, by R&D Systems (2 mentions) Sb431542, by R&D Systems (2 mentions) Rabbit anti fibronectin antibody, by Merck Group (1 mentions) Mouse anti vimentin antibody, by Merck Group (1 mentions) Rabbit anti p smad3, by Cell Signaling Technology (1 mentions) Recombinant human tgf β1 protein, by R&D Systems (1 mentions) Mouse anti gapdh, by Merck Group (1 mentions) Alexa488 anti mouse antibody, by Thermo Fisher Scientific (1 mentions) Rabbit anti zo 1 antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti paxillin antibody, by BD (1 mentions) Alexa fluor 568 coupled phalloidin, by Thermo Fisher Scientific (1 mentions) Irdye 680rd goat anti mouse, by LI COR (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Trypsin edta, by Merck Group (1 mentions) Ab28379, by Abcam (1 mentions) Mouse anti tubulin dm1a, by Merck Group (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Ab40854, by Abcam (1 mentions) Rabbit anti psmad3 c25a9, by Cell Signaling Technology (1 mentions)

4 protocols using mouse anti smad2 3

1

Immunofluorescence Analysis of Cell Adhesion

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mouse anti-paxillin antibody was purchased from BD Biosciences (610052) and the corresponding secondary Alexa488 anti-mouse antibody from ThermoFisher Scientific (A-11029). The rabbit anti-fibronectin antibody was obtained from Sigma (F3648) and the corresponding secondary Alexa647 anti-rabbit antibody from ThermoFisher Scientific (A-21245). Alexa Fluor 568-coupled phalloidin was from ThermoFisher Scientific (A12380). Rat anti-E-cadherin antibody was obtained from ThermoFisher Scientific (13-1900), rabbit anti-ZO1 antibody from ThermoFisher Scientific (61-7300), mouse anti-vimentin antibody from Sigma (V2258), mouse anti-SMAD2/3 from BD Biosciences (610842), rabbit anti-p-SMAD3 from Cell Signaling (p-Ser423/425; 9520) and mouse anti-GAPDH from Sigma (G8795). For quantitative immunoblot analysis, secondary antibodies from LI-COR Biosciences, IRDye 680RD Goat anti-Mouse (926-68070) and IRDye 800CW Goat anti-Rabbit (926-32211) were used. DAPI was acquired from Sigma (D9542), recombinant human TGFβ1 protein from R & D Systems (240B-0-10), 16% paraformaldehyd (PFA) from Electron Microscopy Services (15710-S), fatty-acid free BSA from Calbiochem (126575), Trypsin/EDTA from Sigma (T4174), PBS from Gibco (14200-067), and Triton X-100 from Sigma (X-100).
Lotz-Jenne C., Lüthi U., Ackerknecht S., Lehembre F., Fink T., Stritt M., Wirth M., Pavan S., Bill R., Regenass U., Christofori G, & Meyer-Schaller N. (2016). A high-content EMT screen identifies multiple receptor tyrosine kinase inhibitors with activity on TGFβ receptor. Oncotarget, 7(18), 25983-26002.
+ Open protocol
+ Expand
2

TGF-β Signaling Pathway Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant TGF-β (TGF-β3) and the TGF-β type I receptor inhibitor SB431542 were purchased from R&D systems and Sigma-Aldrich (S4317), respectively. The following antibodies were used: mouse anti-FLAG (M2; Sigma-Aldrich), mouse anti-myc (9E10; Oncogene research products), rabbit anti-pSmad3 (C25A9; Cell Signaling), mouse anti-tubulin (DM1A; Sigma-Aldrich), rabbit anti-HDAC1 (2E10; Millipore), mouse anti-Smad2/3 (BD), rabbit anti-Smad3 (ab28379 and ab40854; Abcam), mouse anti-Smad4 (B-8; Santa Cruz), goat anti-Smad4 (AF2097; R&D) and mouse anti-TTF-1 (8G7G3/1; Novus Biologicals).
Isogaya K., Koinuma D., Tsutsumi S., Saito R.A., Miyazawa K., Aburatani H, & Miyazono K. (2014). A Smad3 and TTF-1/NKX2-1 complex regulates Smad4-independent gene expression. Cell Research, 24(8), 994-1008.
+ Open protocol
+ Expand
3

TGF-β Signaling Pathway Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant TGF-β (TGF-β3) and the TGF-β type I receptor inhibitor SB431542 were purchased from R&D systems (Ixonia, WI, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively. The following antibodies were used: rabbit anti-phospho-Smad2 (Cell Signaling Technology, Danvers, MA, USA.), mouse anti-α-tubulin (DM1A [Sigma-Aldrich]), mouse anti-Smad2/3 (BD Biosciences, Franklin Lakes, NJ, USA.), rabbit anti-PARP (Cell Signaling Technology), mouse anti-E-cadherin (BD Biosciences), mouse anti-N-cadherin (BD Biosciences) and rabbit anti-fibronectin (Sigma-Aldrich).
Arase M., Horiguchi K., Ehata S., Morikawa M., Tsutsumi S., Aburatani H., Miyazono K, & Koinuma D. (2014). Transforming growth factor-β-induced lncRNA-Smad7 inhibits apoptosis of mouse breast cancer JygMC(A) cells. Cancer Science, 105(8), 974-982.
+ Open protocol
+ Expand
4

Immunoprecipitation and Immunoblotting of Cell Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins from transfected 293T or stimulated U2987MG cells were extracted in 0.5 % Triton X-100, 0.5 % sodium deoxycolate, 20 mM Tris pH 7.4, 150 mM NaCl, 10 mM EDTA and complete protease inhibitor cocktail (Roche) during 30 min on ice and centrifuged at 13,000 r.p.m. for 10 min at 4 °C to remove insoluble debris. Proteins were analyzed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) or immunoprecipitated with anti-Flag (1 µg), anti-SMAD1 (1 µg), anti-SMAD4 (1 µg), anti-SMAD2/3 (2 µg), or anti-Snail (3 µg) antibodies, and after four washes with lysis buffer, immunocomplexes were resolved by SDS-PAGE and detected by immunoblotting. The antibodies used were: rabbit-anti-Nestin (Millipore, MAB5326), rabbit-anti-phospho-SMAD2 (home-made) [34 (link)], rabbit-anti-SMAD3 (9523), rabbit-anti-phospho-SMAD1/5 (9511), rabbit-anti-SMAD5 (9517), and rabbit-anti-Snail (C15D3) (Cell Signaling); mouse-anti-SMAD1 (ab53745, Abcam); rabbit-anti-GFAP (sc-6171), mouse-anti-SMAD4 (sc-7966), and mouse-anti-GST (sc-138) (Santa Cruz Inc.); mouse-anti-SMAD2 (EB05147, Everest Biotech); mouse-anti-SMAD2/3 (610843, BD Biosciences); mouse-anti-HA (11666606001, Roche); rabbit-anti-GFP (A111222, Invitrogen); and mouse-anti-Flag M5 (F4042, Sigma). Mouse-anti-GAPDH (AM4300, Ambion) was used as loading control in immunoblot analyses.
Caja L., Tzavlaki K., Dadras M.S., Tan E.J., Hatem G., Maturi N.P., Morén A., Wik L., Watanabe Y., Savary K., Kamali-Moghaddan M., Uhrbom L., Heldin C.H, & Moustakas A. (2018). Snail regulates BMP and TGFβ pathways to control the differentiation status of glioma-initiating cells. Oncogene, 37(19), 2515-2531.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!