Xmap technology

Manufactured by Bio-Rad

Sourced in United States

XMAP technology is a bead-based multiplexed assay platform developed by Bio-Rad. It enables the simultaneous detection and quantification of multiple analytes in a single sample. The technology utilizes color-coded magnetic beads that are coated with specific capture molecules, allowing for the concurrent measurement of numerous target molecules in a sample.

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Lab products found in correlation

Luminex system, by Bio-Rad (2 mentions) Luminex is 100, by Bio-Rad (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions) Bio plex pro human diabetes assay panel, by Bio-Rad (1 mentions) Bio plex pro human cytokine 27 plex, by Bio-Rad (1 mentions) Bio plex multiplex immunoassay, by Bio-Rad (1 mentions) Bio plex manager software v 6, by Bio-Rad (1 mentions)

Close spelling variants of this product

Luminex xMAP technology (55 citations) Luminex xMAP® multiplex technology (2 citations)

7 protocols using xmap technology

Cytokine and Chemokine Quantification

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Cell culture supernatants (100 µl) were harvested 18 hours after stimulation with Vacc-4x peptides and stored at −70°C. Cytokines and chemokines were measured using Bio-Plex XMap technology with Luminex IS 100 (BIO-RAD, CA) and Bio-Plex manager Software v6. StatLIA software package v3 (Brendan Scientific Inc., Carlsbad, CA) was used to calculate sample concentrations.

Brekke K., Lind A., Holm-Hansen C., Haugen I.L., Sørensen B., Sommerfelt M, & Kvale D. (2014). Intranasal Administration of a Therapeutic HIV Vaccine (Vacc-4x) Induces Dose-Dependent Systemic and Mucosal Immune Responses in a Randomized Controlled Trial. PLoS ONE, 9(11), e112556.

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Dysregulated Immune Biomarkers in Thalassemia

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Concentration of cytokines (IL-2, IL-6, IL-10, IL-17A, and TNF- alpha), chemokines (IL-8, macrophage inflammatory protein (MIP)-1alpha), adipocytokines (leptin and adiponectin), growth factor (VEGF), and adhesion molecules (intercellular adhesion molecule (ICAM)-1 and VCAM-1) were determined in plasma obtained from 47 patients with thalassemia, and from 23 healthy subjects (CTR).
Commercially available multiplex magnetic immunoassay kits (Bio-Rad Laboratories, Hercules, CA) were used to quantify concentrations of human IL-2, IL-6, IL-8, IL-10, IL-17A, MIP-1alpha, TNF-alpha, VEGF (Bio-Plex Pro Human Cytokine 8-plex customized from 27-plex), leptin and adiponectin (Bio-Plex Pro Human Diabetes Assay Panel), ICAM-1 and VCAM-1 (Bio-PlexHuman Cytokine Group II 21-Plex Panel, ICAM-1 and VCAM-1).
The multi-analyte suspension array is based on Luminex’s XMAP Technology. The different analytes were quantified using the Bio-Plex protein array reader Bio-Plex^® 200 System (Bio-Rad) equipped with a magnetic washer, and analyzed by using a BioPlex Manager Software version 6.1.
Results were measured and expressed as the concentration of analytes (pg/ml). Values presenting a coefficient of variation beyond 8% were discarded before the final data analysis.

Caprari P., Profumo E., Massimi S., Buttari B., Riganò R., Regine V., Gabbianelli M., Rossi S., Risoluti R., Materazzi S., Gullifa G., Maffei L, & Sorrentino F. (2023). Hemorheological profiles and chronic inflammation markers in transfusion-dependent and non-transfusion- dependent thalassemia. Frontiers in Molecular Biosciences, 9, 1108896.

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Multiplex Immune Analyte Profiling

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Plasma concentrations of the following immune analytes were determined using the bead-based Luminex system based on xMAP technology (Bio-Rad Laboratory, Hercules, CA, USA). Cytokines: interleukin (IL)-1β, IL-1rα, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, IFNγ, tumor necrosis factor (TNF)-α. Chemokines: C-C motif ligand (CCL)2, CCL3, CCL4, CCL5, and CCL11 and C-X-C motif ligand (CXCL) 10. Growth factors: basic fibroblast growth factor (bFGF), granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), platelet-derived growth factor beta (PDGF-β), and vascular endothelial growth factor (VEGF).
Assays were performed on Luminex 200 system. Samples were analyzed according to manufacturer's instructions. The intra-assay coefficient of variation was 2.3–4.8%, and inter-assay coefficient of variation was 4.9–28.2%.

Poletti S., Mazza M.G., Vai B., Lorenzi C., Colombo C, & Benedetti F. (2020). Proinflammatory Cytokines Predict Brain Metabolite Concentrations in the Anterior Cingulate Cortex of Patients With Bipolar Disorder. Frontiers in Psychiatry, 11, 590095.

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Multiplex Immune Analyte Profiling

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Plasma concentrations of the following immune analytes were determined using the Bio-Plex Pro Human Cytokine 27-plex using the bead-based Luminex system based on xMAP technology (Bio-Rad Laboratory, Hercules, CA, USA): Cytokines: Interleukin (IL)-1β, IL-1rα, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, Interferon (IFN)γ, Tumor Necrosis Factor (TNF)α; Chemokines: C–C motif ligand 1 (CCL2), CCL3, CCL4, CCL5, CCL11,; C-X-C motif chemokine (CXCL)10; Growth factors: fibroblast growth factor (FGF) basic, Granulocyte Colony Stimulating Factor (G-CSF), Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), Platelet-Derived Growth Factor Beta (PDGF-B), Vascular Endothelial Growth Factor (VEGF). Lower limits of quantification (LLOQ) and coefficients of variations (CVs) are shown in Table 1_suppl. Assays were performed on Luminex 200 system. Samples were analyzed according to manufacturer's instructions. The intra-assay CV was 2.3–4.8%, inter-assay CV was 4.9–28.2%.

Poletti S., Paolini M., Ernst J., Bollettini I., Melloni E., Vai B., Harrington Y., Bravi B., Calesella F., Lorenzi C., Zanardi R, & Benedetti F. (2022). Long-term effect of childhood trauma: Role of inflammation and white matter in mood disorders. Brain, Behavior, & Immunity - Health, 26, 100529.

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Targeted Proteomic Analysis of Plasma Cytokines

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Fasting blood samples were collected from an antecubital vein, processed to plasma and stored according to protocols (supplementary material). A targeted proteomic panel of 48 cytokines, chemokines and growth factors (table 1 and supplementary table E1) was measured for each subject using Bio-Plex multiplex immunoassay (BioRad, Hercules, CA, USA), a bead-based flow cytometric platform built on Luminex xMAP technology (Austin, TX, USA), according to manufacturer's instructions (supplementary material). Each sample was measured in duplicate. A Luminex 200 plate reader quantified median fluorescence intensity (MFI) for each protein. MFI was used for all analyses rather than standard curve-derived absolute concentrations, as MFI does not require detection limit censoring and has better downstream statistical power [8 (link)]. The inter-assay coefficient of variation was <15% for all measured analytes, based on internal controls included across runs.

Amsallem M., Sweatt A.J., Arthur Ataam J., Guihaire J., Lecerf F., Lambert M., Ghigna M.R., Ali M.K., Mao Y., Fadel E., Rabinovitch M., de Jesus Perez V., Spiekerkoetter E., Mercier O., Haddad F, & Zamanian R.T. (2021). Targeted proteomics of right heart adaptation to pulmonary arterial hypertension. The European Respiratory Journal, 57(4), 2002428.

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Multiplex Microorganism Detection using xMAP

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A multi-analyte profiling (xMAP) technology (Bio-Rad^®, Hercules, CA, USA) was performed according to the Bio-Rad manuals, guides, and protocols. Briefly, magnetic beads were first coupled with specific capture bioreagents (antibody or aptamer), and a sandwich strategy was performed to detect the microorganisms. For this, analytes were captured with a bead-antibody complex, and a biotinylated antibody (Table 1) conjugated with Bio-Plex Streptavidin-PE was used to detect the presence of the analytes.

Moteshareie H., Hassen W.M., Dirieh Y., Groulx E., Dubowski J.J, & Tayabali A.F. (2022). Rapid, Sensitive, and Selective Quantification of Bacillus cereus Spores Using xMAP Technology. Microorganisms, 10(7), 1408.

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Multiplex Cytokine Profiling in Plasma

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Soluble cytokines representing Th1 (TNF-α, INF-γ) and Th2 (IL-4, IL-5, IL-10 and IL-13) profiles were measured in snap-frozen EDTA plasma (see above) using Bio-Plex XMap technology (TX, USA) with a Luminex IS100 instrument (BIO-RAD, CA, USA) and Bio-Plex manager Software v6, according to the instructions by the manufacturer.

Lind A., Brekke K., Pettersen F.O., Mollnes T.E., Trøseid M, & Kvale D. (2014). A Parameter for IL-10 and TGF-ß Mediated Regulation of HIV-1 Specific T Cell Activation Provides Novel Information and Relates to Progression Markers. PLoS ONE, 9(1), e85604.

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