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Pe texas red cd45ra

Manufactured by Beckman Coulter

PE-Texas Red-CD45RA is a fluorescent-labeled antibody used in flow cytometry applications to identify and quantify CD45RA-positive cells. It is conjugated with the PE-Texas Red fluorochrome, which can be excited by a 488 nm or 532 nm laser and detected in the Texas Red channel.

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2 protocols using pe texas red cd45ra

1

Fluorescence-Activated Cell Sorting of Hematopoietic Stem and Progenitor Cells

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Fluorescence-activated cell sorting (FACS) was performed with the FACSAria sorter (BD Biosciences, San Jose, CA). Monoclonal antibodies for cell surface markers were FITC-CD38 (Beckman Coulter, Fullerton, CA), PE- lineage (Lin) markers28 (link), PE-Cy5- IL3Rα, APC-CD90, APC-Cy7- or PE-Cy7-CD34 (BD Biosciences, San Jose, CA) and PE-Texas Red-CD45RA (Beckman-Coulter). CD38+ and CD38 cells were gated off the total CD34+ population. Lin-CD34+CD38 CD90+hematopoietic stem cells (HSC)21 (link), common myeloid progenitors (CMP) LinCD34+CD38+ IL3Rα+CD45RA , and granulocyte-macrophage progenitors (GMP) Lin-CD34+CD38+ IL3Rα+CD45RA+ were collected (Supplementary Figure 1).29 (link)
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2

Fluorescence-Activated Cell Sorting of Hematopoietic Stem and Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescence-activated cell sorting (FACS) was performed with the FACSAria sorter (BD Biosciences, San Jose, CA). Monoclonal antibodies for cell surface markers were FITC-CD38 (Beckman Coulter, Fullerton, CA), PE- lineage (Lin) markers28 (link), PE-Cy5- IL3Rα, APC-CD90, APC-Cy7- or PE-Cy7-CD34 (BD Biosciences, San Jose, CA) and PE-Texas Red-CD45RA (Beckman-Coulter). CD38+ and CD38 cells were gated off the total CD34+ population. Lin-CD34+CD38 CD90+hematopoietic stem cells (HSC)21 (link), common myeloid progenitors (CMP) LinCD34+CD38+ IL3Rα+CD45RA , and granulocyte-macrophage progenitors (GMP) Lin-CD34+CD38+ IL3Rα+CD45RA+ were collected (Supplementary Figure 1).29 (link)
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